Adaptations in metabolism and protein translation give rise to the Crabtree effect in yeast
Artikel i vetenskaplig tidskrift, 2021

Aerobic fermentation, also referred to as the Crabtree effect in yeast, is a well-studied phenomenon that allows many eukaryal cells to attain higher growth rates at high glucose availability. Not all yeasts exhibit the Crabtree effect, and it is not known why Crabtree-negative yeasts can grow at rates comparable to Crabtree-positive yeasts. Here, we quantitatively compared two Crabtree-positive yeasts, Saccharomyces cerevisiae and Schizosaccharomyces pombe, and two Crabtree-negative yeasts, Kluyveromyces marxianus and Scheffersomyces stipitis, cultivated under glucose excess conditions. Combining physiological and proteome quantification with genome-scale metabolic modeling, we found that the two groups differ in energy metabolism and translation efficiency. In Crabtree-positive yeasts, the central carbon metabolism flux and proteome allocation favor a glucose utilization strategy minimizing proteome cost as proteins translation parameters, including ribosomal content and/or efficiency, are lower. Crabtree-negative yeasts, however, use a strategy of maximizing ATP yield, accompanied by higher protein translation parameters. Our analyses provide insight into the underlying reasons for the Crabtree effect, demonstrating a coupling to adaptations in both metabolism and protein translation.

Yeast

Systems biology

Constraint-based modeling

Crabtree effect

Proteomics

Författare

Carl Malina

Chalmers, Biologi och bioteknik, Systembiologi

Wallenberg Center for Protein Research (WCPR)

Rosemary Brown

Novo Nordisk Foundation Center for Biosustainability

Chalmers, Fysik, Kemisk fysik

Johan Björkeroth

Chalmers, Biologi och bioteknik, Systembiologi

Eduard Kerkhoven

Novo Nordisk Foundation Center for Biosustainability

Chalmers, Biologi och bioteknik, Systembiologi

Jens B Nielsen

Novo Nordisk Foundation Center for Biosustainability

Chalmers, Biologi och bioteknik, Systembiologi

BioInnovation Institute

Danmarks Tekniske Universitet (DTU)

Proceedings of the National Academy of Sciences of the United States of America

0027-8424 (ISSN) 1091-6490 (eISSN)

Vol. 118 51 e2112836118

Ämneskategorier

Biokemi och molekylärbiologi

Bioprocessteknik

Annan industriell bioteknik

DOI

10.1073/pnas.2112836118

PubMed

34903663

Mer information

Senast uppdaterat

2022-01-19