Adaptations in metabolism and protein translation give rise to the Crabtree effect in yeast
Artikel i vetenskaplig tidskrift, 2021

Aerobic fermentation, also referred to as the Crabtree effect in yeast, is a well-studied phenomenon that allows many eukaryal cells to attain higher growth rates at high glucose availability. Not all yeasts exhibit the Crabtree effect, and it is not known why Crabtree-negative yeasts can grow at rates comparable to Crabtree-positive yeasts. Here, we quantitatively compared two Crabtree-positive yeasts, Saccharomyces cerevisiae and Schizosaccharomyces pombe, and two Crabtree-negative yeasts, Kluyveromyces marxianus and Scheffersomyces stipitis, cultivated under glucose excess conditions. Combining physiological and proteome quantification with genome-scale metabolic modeling, we found that the two groups differ in energy metabolism and translation efficiency. In Crabtree-positive yeasts, the central carbon metabolism flux and proteome allocation favor a glucose utilization strategy minimizing proteome cost as proteins translation parameters, including ribosomal content and/or efficiency, are lower. Crabtree-negative yeasts, however, use a strategy of maximizing ATP yield, accompanied by higher protein translation parameters. Our analyses provide insight into the underlying reasons for the Crabtree effect, demonstrating a coupling to adaptations in both metabolism and protein translation.

Constraint-based modeling


Systems biology


Crabtree effect


Carl Malina

Wallenberg Center for Protein Research (WCPR)

Chalmers, Biologi och bioteknik, Systembiologi

Rosemary Brown

Chalmers, Fysik, Kemisk fysik

Novo Nordisk Fonden

Johan Björkeroth

Chalmers, Biologi och bioteknik, Systembiologi

Eduard Kerkhoven

Novo Nordisk Fonden

Chalmers, Biologi och bioteknik, Systembiologi

Jens B Nielsen

Danmarks Tekniske Universitet (DTU)

Chalmers, Biologi och bioteknik, Systembiologi

BioInnovation Institute

Novo Nordisk Fonden

Proceedings of the National Academy of Sciences of the United States of America

0027-8424 (ISSN) 1091-6490 (eISSN)

Vol. 118 51 e2112836118


Biokemi och molekylärbiologi


Annan industriell bioteknik





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