Design and biofabrication of a leaf-inspired vascularized cell-delivery device
Artikel i vetenskaplig tidskrift, 2022

We designed and biofabricated a channeled construct as a possible cell-delivery device that can be endothelialized to overcome size limitations due to oxygen diffusion. The channeled device mimicking a leaf was designed using computer-aided design software, with fluid flow through the channels visualized using simulation studies. The device was fabricated either by form casting using a custom 3D-printed plastic mold or by 3D-bioprinting using Pluronic F-127 as sacrificial ink to print the channels. The actual leaf was cast or bioprinted using hydrogel made from a mixture of tunicate cellulose nanofibers and alginate that was cross-linked in calcium chloride solution to allow a stable device. The resulting device was a 20 × 8 × 3 mm or 35 × 18 × 3 mm (length × width × height) leaf with one main channel connected to several side channels. Surface modification using periodate oxidation, followed by laminin bioconjugation, was performed to enhance endothelial cell adhesion in the channels. We subsequently used human umbilical vein endothelial cells to demonstrate the efficacy of the device for promoting endothelialization. These results indicated that the biofabricated device has great potential for use in tissue-engineering for various applications associated with the need of perfusable vasculature.


Biomimetic vascularized cell-delivery device

Mold casting

Nanocellulose-alginate hydrogel



Sanna Sämfors

Chalmers, Kemi och kemiteknik, Tillämpad kemi

Essi M. Niemi

Universitetet i Oslo

Oslo universitetssykehus

Kristin Oskarsdotter

Student vid Chalmers

Claudia Villar Egea

Student vid Chalmers

Andreas Mark

Stiftelsen Fraunhofer-Chalmers Centrum för Industrimatematik

Hanne Scholz

Oslo universitetssykehus

Universitetet i Oslo

Paul Gatenholm

Chalmers, Kemi och kemiteknik, Tillämpad kemi

Cellheal AS


2405-8866 (ISSN)

Vol. 26 e00199


Medicinsk apparatteknik

Textil-, gummi- och polymermaterial

Annan medicinsk bioteknologi



Mer information

Senast uppdaterat