Structural and Molecular Characterization of Squalene Synthase Belonging to the Marine Thraustochytrid Species Aurantiochytrium limacinum Using Bioinformatics Approach
Artikel i vetenskaplig tidskrift, 2022

The marine microorganisms thraustochytrids have been explored for their potential in the production of various bioactive compounds, such as DHA, carotenoids, and squalene. Squalene is a secondary metabolite of the triterpenoid class and is known for its importance in various industrial applications. The bioinformatic analysis for squalene synthase (SQS) gene (the first key enzyme in the triterpenoid synthesis pathway), that is prevailing among thraustochytrids, is poorly investigated. In-silico studies combining sequence alignments and bioinformatic tools helped in the preliminary characterization of squalene synthases found in Aurantiochytrium limacinum. The sequence contained highly conserved regions for SQS found among different species indicated the enzyme had all the regions for its functionality. The signal peptide sequence and transmembrane regions were absent, indicating an important aspect of the subcellular localization. Secondary and 3-D models generated using appropriate templates demonstrated the similarities with SQS of the other species. The 3-D model also provided important insights into possible active, binding, phosphorylation, and glycosylation sites.

Squalene synthase

Bioinformatic analysis

Aurantiochytrium

Squalene

Marine thraustochytrids

Författare

Sachin Vyas

Luleå tekniska universitet

Maurizio Bettiga

Bioeconomy Division

Chalmers, Biologi och bioteknik, Industriell bioteknik

Ulrika Rova

Luleå tekniska universitet

P. Christakopoulos

Luleå tekniska universitet

Leonidas Matsakas

Luleå tekniska universitet

Alok Patel

Luleå tekniska universitet

Marine Drugs

1660-3397 (ISSN)

Vol. 20 3 180

Ämneskategorier

Biokemi och molekylärbiologi

Mikrobiologi

Bioinformatik och systembiologi

DOI

10.3390/md20030180

PubMed

35323479

Mer information

Senast uppdaterat

2022-03-24