Quantification of Microbial Robustness in Yeast
Artikel i vetenskaplig tidskrift, 2022

Stable cell performance in a fluctuating environment is essential for sustainable bioproduction and synthetic cell functionality; however, microbial robustness is rarely quantified. Here, we describe a high-throughput strategy for quantifying robustness of multiple cellular functions and strains in a perturbation space. We evaluated quantification theory on experimental data and concluded that the mean-normalized Fano factor allowed accurate, reliable, and standardized quantification. Our methodology applied to perturbations related to lignocellulosic bioethanol production showed that the industrial bioethanol producing strain Saccharomyces cerevisiae Ethanol Red exhibited both higher and more robust growth rates than the laboratory strain CEN.PK and industrial strain PE-2, while a more robust product yield traded off for lower mean levels. The methodology validated that robustness is function-specific and characterized by positive and negative function-specific trade-offs. Systematic quantification of robustness to end-use perturbations will be important to analyze and construct robust strains with more predictable functions.

yeast

Fano factor

robustness quantification

high-throughput

phenomics

bioprocess

Författare

Cecilia Trivellin

Chalmers, Biologi och bioteknik, Industriell bioteknik

Lisbeth Olsson

Chalmers, Biologi och bioteknik, Industriell bioteknik

Peter Rugbjerg

Chalmers, Biologi och bioteknik, Industriell bioteknik

Enduro Genetics ApS

ACS Synthetic Biology

2161-5063 (eISSN)

Vol. In Press

Ämneskategorier

Produktionsteknik, arbetsvetenskap och ergonomi

Bioprocessteknik

Biomedicinsk laboratorievetenskap/teknologi

DOI

10.1021/acssynbio.1c00615

PubMed

35276039

Mer information

Senast uppdaterat

2022-04-04