Identification of novel arsenic resistance genes in yeast
Artikel i vetenskaplig tidskrift, 2022

Arsenic is a toxic metalloid that affects human health by causing numerous diseases and by being used in the treatment of acute promyelocytic leukemia. Saccharomyces cerevisiae (budding yeast) has been extensively utilized to elucidate the molecular mechanisms underlying arsenic toxicity and resistance in eukaryotes. In this study, we applied a genomic DNA overexpression strategy to identify yeast genes that provide arsenic resistance in wild-type and arsenic-sensitive S. cerevisiae cells. In addition to known arsenic-related genes, our genetic screen revealed novel genes, including PHO86, VBA3, UGP1, and TUL1, whose overexpression conferred resistance. To gain insights into possible resistance mechanisms, we addressed the contribution of these genes to cell growth, intracellular arsenic, and protein aggregation during arsenate exposure. Overexpression of PHO86 resulted in higher cellular arsenic levels but no additional effect on protein aggregation, indicating that these cells efficiently protect their intracellular environment. VBA3 overexpression caused resistance despite higher intracellular arsenic and protein aggregation levels. Overexpression of UGP1 led to lower intracellular arsenic and protein aggregation levels while TUL1 overexpression had no impact on intracellular arsenic or protein aggregation levels. Thus, the identified genes appear to confer arsenic resistance through distinct mechanisms but the molecular details remain to be elucidated.

Pho86

arsenate

Ugp1

arsenite

Tul1

Vba3

Författare

Esin Isik

İzmir Yüksek Teknoloji Enstitüsü (IZ -TECH)

Cigdem Balkan

İzmir Yüksek Teknoloji Enstitüsü (IZ -TECH)

Vivien Karl

Göteborgs universitet

Huseyin Caglar Karakaya

İzmir Yüksek Teknoloji Enstitüsü (IZ -TECH)

Sansan Hua

Göteborgs universitet

Sebastien Rauch

Chalmers, Arkitektur och samhällsbyggnadsteknik, Vatten Miljö Teknik

Markus J. Tamas

Göteborgs universitet

Ahmet Koc

Inonu Universitesi

İzmir Yüksek Teknoloji Enstitüsü (IZ -TECH)

MicrobiologyOpen

2045-8827 (ISSN)

Vol. 11 3 e1284

Ämneskategorier

Mikrobiologi

Mikrobiologi inom det medicinska området

DOI

10.1002/mbo3.1284

Mer information

Senast uppdaterat

2022-05-12