Time of Flight Secondary Ion Mass Spectrometry imaging for precise localization of zirconium-labelled trastuzumab in xenograft cancer tumour tissues
Artikel i vetenskaplig tidskrift, 2022
The human epidermal growth factor receptor 2 (HER2) specific radiotracer zirconium-Desferrioxamine(DFO)-trastuzumab was visualized ex-vivo by Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS) imaging in ovarian and breast cancer xenograft tumor sections. Heterogeneous spatial distribution of [90Zr+] ions re-flected the heterogeneous localization of trastuzumab, observed in parallel by immunohistochemistry staining in HER2+ tumors. Our results show that ToF-SIMS imaging is a quick and sensitive technique to image zirconium labelled biologics at microscale in tissues.
Zirconium-89
Secondary ion mass spectrometry SIMS
Mass spectrometry imaging
Författare
Florent Penen
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Rene Raave
Radboud Universiteit
Annemarie Kip
Radboud Universiteit
Sandra Heskamp
Radboud Universiteit
Per Malmberg
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Microchemical Journal
0026-265X (ISSN)
Vol. 181 107860Ämneskategorier
Medicinsk laboratorie- och mätteknik
Radiologi och bildbehandling
Medicinsk bildbehandling
DOI
10.1016/j.microc.2022.107860