Single-Molecule Trapping and Measurement in a Nanostructured Lipid Bilayer System
Artikel i vetenskaplig tidskrift, 2022

The repulsive electrostatic force between a biomolecule and a like-charged surface can be geometrically tailored to create spatial traps for charged molecules in solution. Using a parallel-plate system composed of silicon dioxide surfaces, we recently demonstrated single-molecule trapping and high precision molecular charge measurements in a nanostructured free energy landscape. Here we show that surfaces coated with charged lipid bilayers provide a system with tunable surface properties for molecular electrometry experiments. Working with molecular species whose effective charge and geometry are well-defined, we demonstrate the ability to quantitatively probe the electrical charge density of a supported lipid bilayer. Our findings indicate that the fraction of charged lipids in nanoslit lipid bilayers can be significantly different from that in the precursor lipid mixtures used to generate them. We also explore the temporal stability of bilayer properties in nanofluidic systems. Beyond their relevance in molecular measurement, such experimental systems offer the opportunity to examine lipid bilayer formation and wetting dynamics on nanostructured surfaces.

Författare

Maria Bespalova

University of Oxford

Robin Öz

Chalmers, Biologi och bioteknik, Kemisk biologi

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi

Madhavi Krishnan

The Kavli Institute for Nanoscience Discovery

University of Oxford

Langmuir

07437463 (ISSN) 15205827 (eISSN)

Vol. 38 45 13923-13934

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Ämneskategorier

Fysikalisk kemi

Annan kemi

Teoretisk kemi

DOI

10.1021/acs.langmuir.2c02203

PubMed

36326814

Mer information

Senast uppdaterat

2024-03-07