Skin permeation studies of chromium species – Evaluation of a reconstructed human epidermis model
Artikel i vetenskaplig tidskrift, 2023

A reconstructed human epidermis (RHE) model, the EpiDerm, was investigated and compared to human skin ex vivo regarding tissue penetration and distribution of two chromium species, relevant in both occupational and general exposure in the population. Imaging mass spectrometry was used in analysis of the sectioned tissue. The RHE model gave similar results compared to human skin ex vivo for skin penetration of CrVI. However, the penetration of CrIII into the tissue of the RHE model compared to human skin ex vivo differed markedly, such that in the RHE model the CrIII species accumulated in the tissue layer corresponding to stratum corneum whereas in human skin ex vivo, the CrIII species penetrated evenly through the skin tissue. Further, skin lipids such as cholesterol were less abundant in the RHE model compared to the human skin tissue. Results presented here indicate that the RHE models do not possess the same fundamental properties as human skin tissue. As the RHE models appear to be able to give false negative results, experiments using RHE models for the study of skin penetration should be evaluated with caution.

Författare

Lina Hagvall

Lunds universitet

Göteborgs universitet

Marwa Munem

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Thuy Mai Hoang Philipsen

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Masoumeh Dowlatshahi Pour

Göteborgs universitet

Y. Hedberg

Western University

Lawson Health Research Institute

Per Malmberg

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Toxicology in Vitro

0887-2333 (ISSN) 18793177 (eISSN)

Vol. 91 105636

Chemical imaging analysis of skin permeation of sensitizing and toxic metals

Insamlingsstiftelsen Hudfonden (3037), 2020-07-01 -- 2021-12-31.

Ämneskategorier

Dermatologi och venereologi

DOI

10.1016/j.tiv.2023.105636

PubMed

37380007

Mer information

Senast uppdaterat

2023-07-19