3D printed inserts for reproducible high throughput screening of cell migration
Artikel i vetenskaplig tidskrift, 2023

Cell migration is a fundamental and complex phenomenon that occurs in normal physiology and in diseases like cancer. Hence, understanding cell migration is very important in the fields of developmental biology and biomedical sciences. Cell migration occurs in 3 dimensions (3D) and involves an interplay of migrating cell(s), neighboring cells, extracellular matrix, and signaling molecules. To understand this phenomenon, most of the currently available techniques still rely on 2-dimensional (2D) cell migration assay, also known as the scratch assay or the wound healing assay. These methods suffer from limited reproducibility in creating a cell-free region (a scratch or a wound). Mechanical/heat related stress to cells is another issue which hampers the applicability of these methods. To tackle these problems, we developed an alternative method based on 3D printed biocompatible cell inserts, for quantifying cell migration in 24-well plates. The inserts were successfully validated via a high throughput assay for following migration of lung cancer cell line (A549 cell line) in the presence of standard cell migration promoters and inhibitors. We also developed an accompanying image analysis pipeline which demonstrated that our method outperforms the state-of-the-art methodologies for assessing the cell migration in terms of reproducibility and simplicity.

cell migration assay

scratch assay

cell migration

wound healing assay

biocompatible cell inserts

3D printing

image analysis

in vitro studies


Abhayraj S. Joshi

Novo Nordisk Fonden

Mukil Madhusudanan

Novo Nordisk Fonden

Ivan Mijakovic

Chalmers, Life sciences, Systembiologi

Novo Nordisk Fonden

Frontiers in Cell and Developmental Biology

2296634X (eISSN)

Vol. 11 1256250

Graphene-based drug delivery systems for treating MRSA infections

NordForsk (105121), 2021-01-01 -- 2023-12-31.



Cell- och molekylärbiologi





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