Mimicking Nonribosomal Peptides from the Marine Actinomycete Streptomyces sp. H-KF8 Leads to Antimicrobial Peptides
Artikel i vetenskaplig tidskrift, 2024
Microorganisms within the marine environment have been shown to be very effective sources of naturally produced antimicrobial peptides (AMPs). Several nonribosomal peptides were identified based on genome mining predictions of Streptomyces sp. H-KF8, a marine Actinomycetota isolated from a remote Northern Chilean Patagonian fjord. Based on these predictions, a series of eight peptides, including cyclic peptides, were designed and chemically synthesized. Six of these peptides showed antimicrobial activity. Mode of action studies suggest that two of these peptides potentially act on the cell membrane via a novel mechanism allowing the passage of small ions, resulting in the dissipation of the membrane potential. This study shows that though structurally similar peptides, determined by NMR spectroscopy, the incorporation of small sequence mutations results in a dramatic influence on their bioactivity including mode of action. The qualified hit sequence can serve as a basis for more potent AMPs in future studies.
antimicrobial peptides
secondary metabolite
marine sediments
Streptomyces
cyclic peptides
nonribosomal peptides
Författare
Luisa I. Beyer
Göteborgs universitet
Ann-Britt Schäfer
Göteborgs universitet
Chalmers, Life sciences, Kemisk biologi
Agustina Undabarrena
Universidad Técnica Federico Santa María
Inger Mattsby-Baltzer
Göteborgs universitet
Daniel Tietze
Göteborgs universitet
Elin Svensson
Chalmers, Life sciences, Kemisk biologi
Alexandra Stubelius
Chalmers, Life sciences, Kemisk biologi
Michaela Wenzel
Chalmers, Life sciences, Kemisk biologi
Göteborgs universitet
Beatriz Cámara
Universidad Técnica Federico Santa María
Alesia A. Tietze
Göteborgs universitet
ACS Infectious Diseases
2373-8227 (eISSN)
Vol. 10 1 79-92Ämneskategorier
Mikrobiologi
DOI
10.1021/acsinfecdis.3c00206
PubMed
38113038