Structural and biochemical analysis of family 92 carbohydrate-binding modules uncovers multivalent binding to β-glucans
Artikel i vetenskaplig tidskrift, 2024
Carbohydrate-binding modules (CBMs) are non-catalytic proteins found appended to carbohydrate-active enzymes. Soil and marine bacteria secrete such enzymes to scavenge nutrition, and they often use CBMs to improve reaction rates and retention of released sugars. Here we present a structural and functional analysis of the recently established CBM family 92. All proteins analysed bind preferentially to β−1,6-glucans. This contrasts with the diversity of predicted substrates among the enzymes attached to CBM92 domains. We present crystal structures for two proteins, and confirm by mutagenesis that tryptophan residues permit ligand binding at three distinct functional binding sites on each protein. Multivalent CBM families are uncommon, so the establishment and structural characterisation of CBM92 enriches the classification database and will facilitate functional prediction in future projects. We propose that CBM92 proteins may cross-link polysaccharides in nature, and might have use in novel strategies for enzyme immobilisation.
Författare
Meng Shu Hao
Alba Nova Universitetscentrum
Zhejiang University
Scott Mazurkewich
Chalmers, Life sciences, Industriell bioteknik
Wallenberg Wood Science Center (WWSC)
He Li
Alba Nova Universitetscentrum
Alma Kvammen
Alba Nova Universitetscentrum
Srijani Saha
Alba Nova Universitetscentrum
Salla Koskela
Alba Nova Universitetscentrum
Wallenberg Wood Science Center (WWSC)
Annie R. Inman
Alba Nova Universitetscentrum
Masahiro Nakajima
Tokyo University of Science
Nobukiyo Tanaka
Tokyo University of Science
Hiroyuki Nakai
Niigata University
Gisela Brändén
Göteborgs universitet
Vincent Bulone
Alba Nova Universitetscentrum
Flinders University
Johan Larsbrink
Chalmers, Life sciences, Industriell bioteknik
Wallenberg Wood Science Center (WWSC)
Lauren S McKee
Alba Nova Universitetscentrum
Wallenberg Wood Science Center (WWSC)
Nature Communications
2041-1723 (ISSN) 20411723 (eISSN)
Vol. 15 1 3429Ämneskategorier
Biokemi och molekylärbiologi
Bioprocessteknik
Strukturbiologi
DOI
10.1038/s41467-024-47584-y
PubMed
38653764