Enhanced upgrading of lignocellulosic substrates by coculture of Saccharomyces cerevisiae and Acinetobacter baylyi ADP1
Artikel i vetenskaplig tidskrift, 2024
Lignocellulosic biomass as feedstock has a huge potential for biochemical production. Still, efficient utilization of hydrolysates derived from lignocellulose is challenged by their complex and heterogeneous composition and the presence of inhibitory compounds, such as furan aldehydes. Using microbial consortia where two specialized microbes complement each other could serve as a potential approach to improve the efficiency of lignocellulosic biomass upgrading.
Results
This study describes the simultaneous inhibitor detoxification and production of lactic acid and wax esters from a synthetic lignocellulosic hydrolysate by a defined coculture of engineered Saccharomyces cerevisiae and Acinetobacter baylyi ADP1. A. baylyi ADP1 showed efficient bioconversion of furan aldehydes present in the hydrolysate, namely furfural and 5-hydroxymethylfurfural, and did not compete for substrates with S. cerevisiae, highlighting its potential as a coculture partner. Furthermore, the remaining carbon sources and byproducts of S. cerevisiae were directed to wax ester production by A. baylyi ADP1. The lactic acid productivity of S. cerevisiae was improved approximately 1.5-fold (to 0.41 ± 0.08 g/L/h) in the coculture with A. baylyi ADP1, compared to a monoculture of S. cerevisiae.
Conclusion
The coculture of yeast and bacterium was shown to improve the consumption of lignocellulosic substrates and the productivity of lactic acid from a synthetic lignocellulosic hydrolysate. The high detoxification capacity and the ability to produce high-value products by A. baylyi ADP1 demonstrates the strain to be a potential candidate for coculture to increase production efficiency and economics of S. cerevisiae fermentations.
Detoxifcation
Cocultivation
Wax esters
Acinetobacter baylyi ADP1
Lactic acid
Saccharomyces cerevisiae
Lignocellulose
Författare
Changshuo Liu
Tampereen Yliopisto
BoHyun Choi
Chalmers, Life sciences, Industriell bioteknik
Elena Efimova
Tampereen Yliopisto
Yvonne Nygård
Chalmers, Life sciences, Industriell bioteknik
Suvi Santala
Tampereen Yliopisto
Biotechnology for Biofuels and Bioproducts
27313654 (eISSN)
Vol. 17 1 61Bioplaster och vaxestrar från träråvara genom samodling av bakterier och jäst
Formas (2019-02510), 2020-04-01 -- 2022-03-31.
J. Gust. Richert stiftelse (2022-0073), 2023-01-01 -- 2023-12-31.
Drivkrafter
Hållbar utveckling
Ämneskategorier
Biokemikalier
Mikrobiologi
Fundament
Grundläggande vetenskaper
DOI
10.1186/s13068-024-02510-8