Enhanced upgrading of lignocellulosic substrates by coculture of Saccharomyces cerevisiae and Acinetobacter baylyi ADP1
Artikel i vetenskaplig tidskrift, 2024

Background
Lignocellulosic biomass as feedstock has a huge potential for biochemical production. Still, efficient utilization of hydrolysates derived from lignocellulose is challenged by their complex and heterogeneous composition and the presence of inhibitory compounds, such as furan aldehydes. Using microbial consortia where two specialized microbes complement each other could serve as a potential approach to improve the efficiency of lignocellulosic biomass upgrading.

Results
This study describes the simultaneous inhibitor detoxification and production of lactic acid and wax esters from a synthetic lignocellulosic hydrolysate by a defined coculture of engineered Saccharomyces cerevisiae and Acinetobacter baylyi ADP1. A. baylyi ADP1 showed efficient bioconversion of furan aldehydes present in the hydrolysate, namely furfural and 5-hydroxymethylfurfural, and did not compete for substrates with S. cerevisiae, highlighting its potential as a coculture partner. Furthermore, the remaining carbon sources and byproducts of S. cerevisiae were directed to wax ester production by A. baylyi ADP1. The lactic acid productivity of S. cerevisiae was improved approximately 1.5-fold (to 0.41 ± 0.08 g/L/h) in the coculture with A. baylyi ADP1, compared to a monoculture of S. cerevisiae.

Conclusion
The coculture of yeast and bacterium was shown to improve the consumption of lignocellulosic substrates and the productivity of lactic acid from a synthetic lignocellulosic hydrolysate. The high detoxification capacity and the ability to produce high-value products by A. baylyi ADP1 demonstrates the strain to be a potential candidate for coculture to increase production efficiency and economics of S. cerevisiae fermentations.

Detoxifcation

Cocultivation

Wax esters

Acinetobacter baylyi ADP1

Lactic acid

Saccharomyces cerevisiae

Lignocellulose

Författare

Changshuo Liu

Tampereen Yliopisto

BoHyun Choi

Chalmers, Life sciences, Industriell bioteknik

Elena Efimova

Tampereen Yliopisto

Yvonne Nygård

Chalmers, Life sciences, Industriell bioteknik

Suvi Santala

Tampereen Yliopisto

Biotechnology for Biofuels and Bioproducts

27313654 (eISSN)

Vol. 17 1 61

Bioplaster och vaxestrar från träråvara genom samodling av bakterier och jäst

Formas (2019-02510), 2020-04-01 -- 2022-03-31.

J. Gust. Richert stiftelse (2022-0073), 2023-01-01 -- 2023-12-31.

Drivkrafter

Hållbar utveckling

Ämneskategorier

Biokemikalier

Mikrobiologi

Fundament

Grundläggande vetenskaper

DOI

10.1186/s13068-024-02510-8

Mer information

Senast uppdaterat

2024-05-23