Quantifying microbial robustness in dynamic environments using microfluidic single-cell cultivation
Artikel i vetenskaplig tidskrift, 2024
RESULTS: Saccharomyces cerevisiae CEN.PK113-7D, harbouring a biosensor for intracellular ATP levels, was exposed to glucose feast-starvation cycles, with each condition lasting from 1.5 to 48 min over a 20 h period. A semi-automated image and data analysis pipeline was developed and applied to assess the performance and robustness of various functions at population, subpopulation, and single-cell resolution. We observed a decrease in specific growth rate but an increase in intracellular ATP levels with longer oscillation intervals. Cells subjected to 48 min oscillations exhibited the highest average ATP content, but the lowest stability over time and the highest heterogeneity within the population. C
ONCLUSION: The proposed pipeline enabled the investigation of function stability in dynamic environments, both over time and within populations. The strategy allows for parallelisation and automation, and is easily adaptable to new organisms, biosensors, cultivation conditions, and oscillation frequencies. Insights on the microbial response to changing environments will guide strain development and bioprocess optimisation.
Biosensors
Live-cell imaging
Population heterogeneity
Nutrient oscillation
Microfluidic single-cell cultivation
Scale-down
Dynamic environments
Saccharomyces cerevisiae
Författare
Luisa Blöbaum
Universität Bielefeld
Luca Torello Pianale
Chalmers, Life sciences, Industriell bioteknik
Lisbeth Olsson
Chalmers, Life sciences, Industriell bioteknik
Alexander Grünberger
Universität Bielefeld
Karlsruher Institut für Technologie (KIT)
Microbial Cell Factories
14752859 (eISSN)
Vol. 23 1 44-Ämneskategorier (SSIF 2011)
Analytisk kemi
Bioenergi
Mikrobiologi
Styrkeområden
Energi
Hälsa och teknik
DOI
10.1186/s12934-024-02318-z
PubMed
38336674