Taxonomic reliability of DNA sequences in public sequence databases: A fungal perspective
Artikel i vetenskaplig tidskrift, 2006

Background DNA sequences are increasingly seen as one of the primary information sources for species identification in many organism groups. Such approaches, popularly known as barcoding, are underpinned by the assumption that the reference databases used for comparison are sufficiently complete and feature correctly and informatively annotated entries. Methodology/Principal Findings The present study uses a large set of fungal DNA sequences from the inclusive International Nucleotide Sequence Database to show that the taxon sampling of fungi is far from complete, that about 20% of the entries may be incorrectly identified to species level, and that the majority of entries lack descriptive and up-to-date annotations. Conclusions The problems with taxonomic reliability and insufficient annotations in public DNA repositories form a tangible obstacle to sequence-based species identification, and it is manifest that the greatest challenges to biological barcoding will be of taxonomical, rather than technical, nature.

Författare

R. Henrik Nilsson

Göteborgs universitet

Martin Ryberg

Göteborgs universitet

Erik Kristiansson

Göteborgs universitet

Chalmers, Matematiska vetenskaper, matematisk statistik

Kessy Abarenkov

Tartu Ülikool

Karl-Henrik Larsson

Göteborgs universitet

Urmas Kõljalg

Tartu Ülikool

PLoS ONE

1932-6203 (ISSN)

Vol. 1 1 e59-

Ämneskategorier

Biologiska vetenskaper

DOI

10.1371/journal.pone.0000059