Bioimaging TOF-SIMS of tissues by gold ion bombardment of a silver-coated thin section.
Artikel i vetenskaplig tidskrift, 2004

The imaging time-of-flight secondary-ion-mass-spectrometry (TOF-SIMS) method was utilized to address the problem of cholesterol localization in rat tissues. Rat kidneys were fixed, cryoprotected by sucrose, frozen, sectioned by cryoultramicrotomy, and dried at room temperature. The samples were either covered with a thin silver layer or analyzed uncovered in an imaging TOF-SIMS instrument equipped with an Au1-3(+)-source. The yield of desorbed secondary ions for some species was up to 600-fold higher after silver coating of the samples. Reference samples of cholesterol were silver-coated and analyzed by TOF-SIMS to define significant peaks, specific for cholesterol. Such peaks were found at m/z = 386 (C27H46O+), m/z = 493 (C27H46O107Ag+), m/z = 495 (C27H46O109Ag+), m/z = 879 (C54H92O2 107Ag+), and m/z = 881 (C54H92O2 109Ag+). The silver-cationized cholesterol (493 < or = m/z < or = 495) signal was localized by imaging TOF-SIMS in the kidney sections and showed a high cholesterol content in the kidney glomeruli. A more diffuse distribution of cholesterol was also found over areas representing the cytoplasm or plasma membrane of the epithelial cells in the proximal tubules of rat kidney.

Scanning

Rats

Gold

Microscopy

Cholesterol

Microtomy

chemistry

analysis

Male

Spectrometry

Rats

Animals

Kidney

Silver

Electron

Sprague-Dawley

Secondary Ion

Mass

Författare

Håkan Nygren

Göteborgs universitet

Bengt R Johansson

Göteborgs universitet

Per Malmberg

Göteborgs universitet

Microscopy Research and Technique

1059-910X (ISSN)

Vol. 65 282-6

Ämneskategorier

MEDICIN OCH HÄLSOVETENSKAP

DOI

10.1002/jemt.20133

PubMed

15662659