A Lipidic-Sponge Phase Screen for Membrane Protein Crystallization
Artikel i vetenskaplig tidskrift, 2008

A major current deficit in structural biology is the lack of high-resolution structures of eukaryotic membrane proteins, many of which are key drug targets for the treatment of disease. Numerous eukaryotic membrane proteins require specific lipids for their stability and activity, and efforts to crystallize and solve the structures of membrane proteins that do not address the issue of lipids frequently end in failure rather than success. To help address this problem, we have developed a sparse matrix crystallization screen consisting of 48 lipidic-sponge phase conditions. Sponge phases form liquid lipid bilayer environments which are suitable for conventional hanging- and sitting-drop crystallization experiments. Using the sponge phase screen, we obtained crystals of several different membrane proteins from bacterial and eukaryotic sources. We also demonstrate how the screen may be manipulated by incorporating specific lipids such as cholesterol; this modification led to crystals being recovered from a bacterial photosynthetic core complex.

Författare

Annemarie Wöhri

Chalmers, Kemi- och bioteknik, Molekylär mikroskopi

Linda C Johansson

Göteborgs universitet

Pia Jeanette Hindrichsen

Chalmers, Kemi- och bioteknik, Farmaceutisk teknologi

Weixiao Yuan Wahlgren

Göteborgs universitet

Gerhard Fischer

Göteborgs universitet

Rob Horsefield

Göteborgs universitet

Gergely Katona

Göteborgs universitet

Anna Maria Nyblom

Chalmers, Kemi- och bioteknik, Molekylär mikroskopi

Fredrik Öberg

Göteborgs universitet

G Young

Glasgow Biomedical Reseach Centre

Richard J Cogdell

Glasgow Biomedical Reseach Centre

N J Fraser

Glasgow Biomedical Reseach Centre

Sven Engström

Chalmers, Kemi- och bioteknik, Teknisk ytkemi

Richard Neutze

Göteborgs universitet

Structure

0969-2126 (ISSN)

Vol. 16 7 1003-1009

Ämneskategorier

Biokemi och molekylärbiologi

Kemi

DOI

10.1016/j.str.2008.06.003