A chemical waveform synthesizer
Artikel i vetenskaplig tidskrift, 2005

Algorithms and methods were developed to synthesize complex chemical waveforms in open volumes by using a scanning-probe microfluidic platform. Time-dependent variations and oscillations of one or several chemical species around the scanning probe, such as formation of sine waves, damped oscillations, and generation of more complex patterns, are demonstrated. Furthermore, we show that intricate bursting and chaotic calcium oscillations found in biological microdomains can be reproduced and that a biological cell can be used as a probe to study receptor functionalities as a function of exposure to time-dependent variations of receptor activators and inhibitors. Thus, the method allows for studies of biologically important oscillatory reactions. More generally, the system allows for detailed studies of complex time-varying chemical and physical phenomena in solution or at solution/surf ace interfaces. © 2005 by The National Academy of Sciences of the USA.

channels

oscillations

cells

receptors

gradients

pulses

solution exchange

microfluidics

signal

neurons

Författare

Jessica Olofsson

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Helen Bridle

Chalmers University of Technology

J. Sinclair

Chalmers University of Technology

D. Granfeldt

Chalmers University of Technology

Eskil Sahlin

Göteborgs universitet

Owe Orwar

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Proceedings of the National Academy of Sciences of the United States of America

0027-8424 (ISSN) 1091-6490 (eISSN)

Vol. 102 8097-8102

Ämneskategorier

Tvärvetenskapliga studier

DOI

10.1073/pnas.0500230102