Towards a biosensor immunoassay of protein-bound isopeptides in human plasma
Artikel i vetenskaplig tidskrift, 2008

This study demonstrates that synthetic isopeptides formed on BSA can be quantitatively analyzed by a surface plasmon resonance-based biosensor method. A monoclonal IgM antibody 81D4, that reacts with the synthetic isopeptide and also with the natural isopeptide cross-link in D-dimer (but not with its non-cross-linked fibrin monomer), was covalently immobilized to a carboxymethylated dextran surface, a CM5 surface. Its immunocapturing efficiency was found to be good. The affinity of the interaction between the monoclonal 81D4 and the synthetic isopeptide was estimated to approximately 4x10(-7)M. Good reactivity was also observed when human plasma spiked with this isopeptide was used as test solution. Cross-linked D-dimer in the plasma of patients is a marker of disseminated intravascular coagulation (DIC) which occurs late in sepsis. This biosensor method has the potential to be developed into a rapid sensitive assay for measuring the level of natural isopeptide cross-links in proteins in the plasma of patients with a suspected diagnosis of sepsis.

Författare

Anna Bergstrand

Chalmers, Kemi- och bioteknik, Teknisk ytkemi

Ismail Ceylan

Gerard Quash

Magnus Nydén

Chalmers, Kemi- och bioteknik, Teknisk ytkemi

SuMo Biomaterials

Krister Holmberg

Chalmers, Kemi- och bioteknik, Teknisk ytkemi

Colloids and Surfaces B: Biointerfaces

0927-7765 (ISSN)

Vol. 66 150-

Ämneskategorier

Kemiteknik

Kemi