Signal frequency studies of an environmental application of a 65 nm region ion sensitive field effect transistor sensor
Artikel i vetenskaplig tidskrift, 2007

A rapid and sensitive novel type of bioelectronic Region Ion Sensitive Field Effect Transistor (RISFET) nanosensor was constructed on a chip with a 65 nm sensing electrode gap. The RISFET nanosensor was demonstrated for the environmental pesticide analysis of neurotoxic organocarbamate/carbofuran. The linear range for carbofuran analysis is ac signal frequency dependent, studied in the range (500 down-0.5 Hz, 50 mV(peak-peak) ac) and a bias voltage applied between the bottom capacitor plate and the electrodes. The signal current response is measured using a low-noise pico ammeter. The inhibition of acetylcholinesterase (AChE) by carbofuran was detectable in a logarithmic linear range (0.1-100nM) at 1.08 Hz, with a lower limit of detection of inhibition 0.1 nM with 10 min incubation time. The sensor is based on the principle of focusing charged reaction products with an electrical field in a region between the sensing electrodes. The current measurement by the sensor electrodes is correlated to the composition of the sample. The carbofuran detection is based on the ability to inhibit the enzyme AChE. The RISFET sensor chip is fabricated using conventional electron beam lithography. The encompassed sensor volume in the "nanocell" is in the attoliter range. (c) 2007 Elsevier B.V. All rights reserved.

Författare

K. Risveden

Lunds universitet

S. Bhand

Birla Institute of Technology and Science Pilani

Lunds universitet

J. F. Ponten

Lunds universitet

T. Anden

Lunds universitet

Nils Calander

Chalmers, Teknisk fysik, Fysikalisk elektronik

Mahdad Sadeghi

Göteborgs universitet

Linköpings universitet

B. Danielsson

Lunds universitet

Sensors and Actuators, B: Chemical

0925-4005 (ISSN)

Vol. 127 1 198-203

Ämneskategorier

Fysik

DOI

10.1016/j.snb.2007.07.046