Lipid imaging of human skeletal muscle using TOF-SIMS with bismuth cluster ion as a primary ion source.
Artikel i vetenskaplig tidskrift, 2008

Intramyocellular lipids are of importance in lipid-related diseases. The techniques in this field are limited because of a lack of adequate tools for localization of various lipids. The most usual methods for the localization of lipid distribution in the skeletal muscle are histochemistry and fluorescence probes. Different chromatography methods and mass spectrometry techniques have also been used for lipid identification. Our aim was to localize the spatial distribution of lipids in their native forms by using static time-of-flight secondary-ion mass spectrometry (TOF-SIMS). Human percutaneous skeletal muscle biopsies were obtained from the middle part of the lateral vastus muscle in the right leg of healthy adolescents with a body mass index >30. Samples were prepared by high-pressure freezing, freeze-fracturing and freeze-drying, and analysed by imaging TOF-SIMS equipped with a Bi3+ cluster ion gun. In the positive spectra, we identified phosphocholine, cholesterol, diacylglycerol, phospholipids and triacylglycerol. Phosphocholine was localized to the edge of the fibre, representing the sarcoplasma or endomysium. Weak cholesterol signals were observed in the intracellular areas. High diacylglycerol and low triacylglycerol signal intensities were seen in intracellular spaces of the transversal area of the muscle fibre. In the negative spectra, we identified fatty acids. We observed co-localization of fatty acids and diacylglycerol, which may indicate lipid-storing parts of the skeletal muscle. Thus, TOF-SIMS imaging can be used to depict the heterogeneous localization of lipids in human skeletal muscle.

analysis

Freeze Drying

methods

Mass

Freeze Fracturing

Secondary Ion

Cholesterol

Adolescent

analysis

analysis

Diglycerides

Microscopy

Phosphorylcholine

analysis

ultrastructure

Fast-Twitch

Slow-Twitch

ultrastructure

chemistry

Fatty Acids

analysis

Scanning

Spectrometry

Humans

Lipids

Muscle Fibers

Quadriceps Muscle

Bismuth

chemistry

Muscle Fibers

ultrastructure

Electron

Triglycerides

chemistry

analysis

Författare

Ylva Magnusson

Göteborgs universitet

Peter Friberg

Göteborgs universitet

Peter Sjövall

Frida Dangardt

Göteborgs universitet

Per Malmberg

Göteborgs universitet

Yun Chen

Göteborgs universitet

Clinical Physiology and Functional Imaging

1475-0961 (ISSN)

Vol. 28 202-9

Ämneskategorier

Cell- och molekylärbiologi

Fysiologi

DOI

10.1111/j.1475-097X.2008.00796.x

PubMed

18363737