Fractionation of processed spruce wood obtained in the production of ethanol
Artikel i vetenskaplig tidskrift, 2009

Fuel ethanol can be produced from pretreated spruce wood through enzymatic hydrolysis and fermentation. Processed spruce wood samples (acid-catalyzed steam hydrolysis followed by enzymatic hydrolysis and fermentation) were fractionated into water-soluble products and residual solids. The dioxane/water soluble portions of the solids were fractionated by liquid-liquid extraction. A substantial portion of the processed wood (20-70 %) was insoluble in both water and dioxane/water. An almost pure lignin fraction, corresponding to about 20 % of the total lignin of the wood, was isolated. Examinations by 1H NMR spectroscopy showed that the processes led to extensive cleavage of arylglycerol ß-aryl ethers in the lignin. The lignin content of other fractions could be estimated very roughly by IR spectroscopy. Purified lignin from SO2-catalyzed steam hydrolysis contained approx. 0.2 % sulphur.

Lignin content

Sulphur content



Enzymatic hydrolysis



Christian Johansson

Knut Lundquist

Chalmers, Kemi- och bioteknik, Skogsindustriell kemiteknik

Hans Theliander

Wallenberg Wood Science Center (WWSC)

Chalmers, Kemi- och bioteknik, Skogsindustriell kemiteknik


1930-2126 (ISSN) 19302126 (eISSN)

Vol. 4 1 15-25


Kemiska processer


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