Image analysis algorithms for cell contour recognition in budding yeast
Artikel i vetenskaplig tidskrift, 2008

Quantification of protein abundance and subcellular localization dynamics from fluorescence microscopy images is of high contemporary interest in cell and molecular biology. For large-scale studies of cell populations and for time-lapse studies, such quantitative analysis can not be performed effectively without some kind of automated image analysis tool. Here, we present fast algorithms for automatic cell contour recognition in bright field images, optimized to the model organism budding yeast (Saccharomyces cerevisiae). The cell contours can be used to effectively quantify cell morphology parameters as well as protein abundance and subcellular localization from overlaid fluorescence data.

Författare

Mats Kvarnström

Chalmers, Teknisk fysik, Bionanofotonik

Katarina Logg

Chalmers, Teknisk fysik, Bionanofotonik

Alfredo Diez

Chalmers, Teknisk fysik, Bionanofotonik

Kristofer Bodvard

Chalmers, Teknisk fysik, Bionanofotonik

Mikael Käll

Chalmers, Teknisk fysik, Bionanofotonik

Optics Express

1094-4087 (ISSN)

Vol. 16 17 12943-12957

Ämneskategorier

Biokemi och molekylärbiologi

Beräkningsmatematik

Atom- och molekylfysik och optik

DOI

10.1364/OE.16.012943