Engineering microporosity in bacterial cellulose scaffolds
Artikel i vetenskaplig tidskrift, 2008

The scaffold is an essential component in tissue engineering. A novel method to prepare threedimensional (3D) nanofibril network scaffolds with controlled microporosity has been developed. By placing paraffin wax and starch particles of various sizes in a growing culture of Acetobacter xylinum, bacterial cellulose scaffolds of different morphologies and interconnectivity were prepared. Paraffin particles were incorporated throughout the scaffold, while starch particles were found only in the outermost area of the resulting scaffold. The porogens were successfully removed after culture with bacteria and no residues were detected with electron spectroscopy for chemical analysis (ESCA) or Fourier transform infra-red spectroscopy (FT-IR). Resulting scaffolds were seeded with smooth muscle cells (SMCs) and investigated using histology and organ bath techniques. SMC were selected as the cell type since the main purpose of the resulting scaffolds is for tissue engineered blood vessels. SMCs attached to and proliferated on and partly into the scaffolds. Copyright © 2008 John Wiley & Sons, Ltd.

Författare

Henrik Bäckdahl

Chalmers, Kemi- och bioteknik, Polymerteknologi

Maricris Esguerra

Sahlgrenska universitetssjukhuset

Dick Delbro

Sahlgrenska universitetssjukhuset

Hogskolan i Kalmar

Bo Risberg

Sahlgrenska universitetssjukhuset

Paul Gatenholm

Chalmers, Kemi- och bioteknik, Polymerteknologi

Journal of tissue engineering and regenerative medicine

1932-6254 (ISSN)

Vol. 2 320-330

Ämneskategorier

Annan medicinsk grundvetenskap

DOI

10.1002/term.97