Investigation of Cancer Cell Behavior on Nanofibrous Scaffolds
Artikel i vetenskaplig tidskrift, 2011

Tissue engineering and the use of nanofibrous biomaterial scaffolds offer a unique perspective for studying cancer development in vitro. Current in vitro models of tumorigenesis are limited by the use of static, two-dimensional (2D) cell culture monolayers that lack the structural architecture necessary for cell-cell interaction and three-dimensional (3D) scaffolds that are too simplistic for studying basic pathological mechanisms. In this study, two nanofibrous biomaterials that mimic the structure of the extracellular matrix, bacterial cellulose and electrospun polycaprolactone (PCL)/collagen I, were investigated as potential 3D scaffolds for an in vitro cancer model. Multiple cancer cell lines were cultured on each scaffold material and monitored for cell viability, proliferation, adhesion, infiltration, and morphology. Both bacterial cellulose and electrospun PCL/collagen I, which have nanoscale structures on the order of 100-500 nm, have been used in many diverse tissue engineering applications. Cancer cell adhesion and growth were limited on bacterial cellulose, while all cellular processes were enhanced on the electrospun scaffolds. This initial analysis has demonstrated the potential of electrospun PCL/collagen I scaffolds toward the development of an improved 3D in vitro cancer model.

Nanofibrous scaffold



Collagen I

Bacterial cellulose



C. S. Szot

Virginia Polytechnic Institute and State University

C Buchanan

Virginia Polytechnic Institute and State University

Paul Gatenholm

Chalmers, Kemi- och bioteknik, Polymerteknologi

M. N. Rylander

Virginia Polytechnic Institute and State University

J Freeman

Virginia Polytechnic Institute and State University

Materials Science and Engineering C

0928-4931 (ISSN) 18730191 (eISSN)

Vol. 31 1 37-42



Industriell bioteknik



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