Nanosized fibers' effect on adult human articular chondrocytes behavior.
Artikel i vetenskaplig tidskrift, 2013
Tissue engineering with chondrogenic cell based therapies is an expanding field with the intention of treating cartilage defects. It has been suggested that scaffolds used in cartilage tissue engineering influence cellular behavior and thus the long-term clinical outcome. The objective of this study was to assess whether chondrocyte attachment, proliferation and post-expansion re-differentiation could be influenced by the size of the fibers presented to the cells in a scaffold. Polylactic acid (PLA) scaffolds with different fiber morphologies were produced, i.e. microfiber (MS) scaffolds as well as nanofiber-coated microfiber scaffold (NMS). Adult human articular chondrocytes were cultured in the scaffolds in vitro up to 28 days, and the resulting constructs were assessed histologically, immunohistochemically, and biochemically. Attachment of cells and serum proteins to the scaffolds was affected by the architecture. The results point toward nano-patterning onto the microfibers influencing proliferation of the chondrocytes, and the overall 3D environment having a greater influence on the re-differentiation. In the efforts of finding the optimal scaffold for cartilage tissue engineering, studies as the current contribute to the knowledge of how to affect and control chondrocytes behavior.
Nanofibers
Particle Size
Cell Differentiation
Cell Adhesion
ultrastructure
Chondrocytes
Chondrogenesis
Cell Count
ultrastructure
Tissue Scaffolds
Adult
Cell Shape
cytology
Cartilage
Articular
Humans
chemistry
Immunohistochemistry
Cell Proliferation
cytology
Författare
Hanna Stenhamre
Chalmers, Kemi- och bioteknik, Polymerteknologi
Anna Thorvaldsson
Chalmers, Kemi- och bioteknik, Polymerteknologi
Lars Enochson
Göteborgs universitet
Pernilla Walkenström
Swerea
Anders Lindahl
Göteborgs universitet
Mats Brittberg
Göteborgs universitet
Paul Gatenholm
Chalmers, Kemi- och bioteknik, Polymerteknologi
Materials Science and Engineering C
0928-4931 (ISSN) 18730191 (eISSN)
Vol. 33 3 1539-1545Ämneskategorier
Materialteknik
Polymerteknologi
DOI
10.1016/j.msec.2012.12.059
PubMed
23827606