Guanine Can Direct Binding Specificity of Ru–dipyridophenazine (dppz) Complexes to DNA through Steric Effects
Artikel i vetenskaplig tidskrift, 2017
X-ray crystal structures of three ?-[Ru(L)2dppz]2+ complexes (dppz=dipyridophenazine; L=1,10-phenanthroline (phen), 2,2?-bipyridine (bpy)) bound to d((5BrC)GGC/GCCG) showed the compounds intercalated at a 5?-CG-3? step. The compounds bind through canted intercalation, with the binding angle determined by the guanine NH2 group, in contrast to symmetrical intercalation previously observed at 5?-TA-3? sites. This result suggests that canted intercalation is preferred at 5?-CG-3? sites even though the site itself is symmetrical, and we hypothesise that symmetrical intercalation in a 5?-CG-3? step could give rise to a longer luminescence lifetime than canted intercalation.
DNA structures
structural biology
nucleic acids
DNA
Författare
James P. Hall
Diamond Light Source
University of Reading
Sarah P. Gurung
Diamond Light Source
University of Reading
Jessica Henle
University of Reading
Patrick Poidl
University of Reading
Johanna Andersson
Chalmers, Kemi och kemiteknik, Tillämpad kemi
Per Lincoln
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Graeme Winter
Diamond Light Source
Thomas Sorensen
Diamond Light Source
David J. Cardin
University of Reading
John A. Brazier
University of Reading
Christine J. Cardin
University of Reading
Chemistry - A European Journal
0947-6539 (ISSN) 1521-3765 (eISSN)
Vol. 23 21 4981-4985Drivkrafter
Hållbar utveckling
Styrkeområden
Nanovetenskap och nanoteknik
Livsvetenskaper och teknik (2010-2018)
Ämneskategorier
Kemi
DOI
10.1002/chem.201605508