Characterization of different fat depots in NAFLD using inflammation-associated proteome, lipidome and metabolome
Artikel i vetenskaplig tidskrift, 2018
Non-alcoholic fatty liver disease (NAFLD) is recognized as a liver manifestation of metabolic syndrome, accompanied with excessive fat accumulation in the liver and other vital organs. Ectopic fat accumulation was previously associated with negative effects at the systemic and local level in the human body. Thus, we aimed to identify and assess the predictive capability of novel potential metabolic biomarkers for ectopic fat depots in non-diabetic men with NAFLD, using the inflammation-associated proteome, lipidome and metabolome. Myocardial and hepatic triglycerides were measured with magnetic spectroscopy while function of left ventricle, pericardial and epicardial fat, subcutaneous and visceral adipose tissue were measured with magnetic resonance imaging. Measured ectopic fat depots were profiled and predicted using a Random Forest algorithm, and by estimating the Area Under the Receiver Operating Characteristic curves. We have identified distinct metabolic signatures of fat depots in the liver (TAG50:1, glutamate, diSM18:0 and CE20:3), pericardium (N-palmitoyl-sphinganine, HGF, diSM18:0, glutamate, and TNFSF14), epicardium (sphingomyelin, CE20:3, PC38:3 and TNFSF14), and myocardium (CE20:3, LAPTGF-β1, glutamate and glucose). Our analyses highlighted non-invasive biomarkers that accurately predict ectopic fat depots, and reflect their distinct metabolic signatures in subjects with NAFLD.
Författare
Alen Lovric
Kungliga Tekniska Högskolan (KTH)
Marit Granér
Helsinki University Central Hospital
Elias Björnson
Wallenberg Lab.
Chalmers, Biologi och bioteknik, Systembiologi
Muhammad Arif
Kungliga Tekniska Högskolan (KTH)
Rui Benfeitas
Kungliga Tekniska Högskolan (KTH)
Kristofer Nyman
Helsinki University Central Hospital
Marcus Ståhlman
Wallenberg Lab.
Markku O. Pentikäinen
Helsinki University Central Hospital
J. Lundbom
Helsinki University Central Hospital
A. Hakkarainen
Helsinki University Central Hospital
Reijo Sirén
City of Helsinki
Markku S. Nieminen
Helsinki University Central Hospital
N. Lundbom
Helsinki University Central Hospital
Kirsi Lauerma
Helsinki University Central Hospital
Marja Riitta Taskinen
Helsinki University Central Hospital
Adil Mardinoglu
Chalmers, Biologi och bioteknik, Systembiologi
Kungliga Tekniska Högskolan (KTH)
Jan Borén
Wallenberg Lab.
Scientific Reports
2045-2322 (ISSN) 20452322 (eISSN)
Vol. 8 1 14200Ämneskategorier
Endokrinologi och diabetes
Annan klinisk medicin
Biologiska vetenskaper
Näringslära
Infrastruktur
Beräkningsinfrastruktur för systembiologi
Styrkeområden
Livsvetenskaper och teknik (2010-2018)
DOI
10.1038/s41598-018-31865-w