Saccharomyces cerevisiae as a Heterologous Host for Natural Products
Kapitel i bok, 2022

Cell factories can provide a sustainable supply of natural products with applications as pharmaceuticals, food-additives or biofuels. Besides being an important model organism for eukaryotic systems, Saccharomyces cerevisiae is used as a chassis for the heterologous production of natural products. Its success as a cell factory can be attributed to the vast knowledge accumulated over decades of research, its overall ease of engineering and its robustness. Many methods and toolkits have been developed by the yeast metabolic engineering community with the aim of simplifying and accelerating the engineering process.In this chapter, a range of methodologies are highlighted, which can be used to develop novel natural product cell factories or to improve titer, rate and yields of an existing cell factory with the goal of developing an industrially relevant strain. The addressed topics are applicable for different stages of a cell factory engineering project and include the choice of a natural product platform strain, expression cassette design for heterologous or native genes, basic and advanced genetic engineering strategies, and library screening methods using biosensors. The many engineering methods available and the examples of yeast cell factories underline the importance and future potential of this host for industrial production of natural products.

Metabolic engineering


Engineering toolkits


Platform strains

Expression cassettes

Cell factory engineering

Natural products

Saccharomyces cerevisiae

Design–Build–Test–Learn Cycle


Maximilian Otto

Chalmers, Biologi och bioteknik, Systembiologi

Novo Nordisk Fonden

Dany Liu

Chalmers, Biologi och bioteknik, Systembiologi

Novo Nordisk Fonden

Verena Siewers

Chalmers, Biologi och bioteknik, Systembiologi

Novo Nordisk Fonden

Methods in Molecular Biology

10643745 (ISSN) 1940-6029 (eISSN)

Vol. 2489 333-367


Produktionsteknik, arbetsvetenskap och ergonomi

Annan maskinteknik

Annan industriell bioteknik





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