Structural and biochemical analysis of family 92 carbohydrate-binding modules uncovers multivalent binding to β-glucans
Artikel i vetenskaplig tidskrift, 2024

Carbohydrate-binding modules (CBMs) are non-catalytic proteins found appended to carbohydrate-active enzymes. Soil and marine bacteria secrete such enzymes to scavenge nutrition, and they often use CBMs to improve reaction rates and retention of released sugars. Here we present a structural and functional analysis of the recently established CBM family 92. All proteins analysed bind preferentially to β−1,6-glucans. This contrasts with the diversity of predicted substrates among the enzymes attached to CBM92 domains. We present crystal structures for two proteins, and confirm by mutagenesis that tryptophan residues permit ligand binding at three distinct functional binding sites on each protein. Multivalent CBM families are uncommon, so the establishment and structural characterisation of CBM92 enriches the classification database and will facilitate functional prediction in future projects. We propose that CBM92 proteins may cross-link polysaccharides in nature, and might have use in novel strategies for enzyme immobilisation.

Författare

Meng Shu Hao

Alba Nova Universitetscentrum

Zhejiang University

Scott Mazurkewich

Chalmers, Life sciences, Industriell bioteknik

Wallenberg Wood Science Center (WWSC)

He Li

Alba Nova Universitetscentrum

Alma Kvammen

Alba Nova Universitetscentrum

Srijani Saha

Alba Nova Universitetscentrum

Salla Koskela

Alba Nova Universitetscentrum

Wallenberg Wood Science Center (WWSC)

Annie R. Inman

Alba Nova Universitetscentrum

Masahiro Nakajima

Tokyo University of Science

Nobukiyo Tanaka

Tokyo University of Science

Hiroyuki Nakai

Niigata University

Gisela Brändén

Göteborgs universitet

Vincent Bulone

Alba Nova Universitetscentrum

Flinders University

Johan Larsbrink

Chalmers, Life sciences, Industriell bioteknik

Wallenberg Wood Science Center (WWSC)

Lauren S McKee

Alba Nova Universitetscentrum

Wallenberg Wood Science Center (WWSC)

Nature Communications

2041-1723 (ISSN) 20411723 (eISSN)

Vol. 15 1 3429

Ämneskategorier

Biokemi och molekylärbiologi

Bioprocessteknik

Strukturbiologi

DOI

10.1038/s41467-024-47584-y

PubMed

38653764

Mer information

Senast uppdaterat

2024-07-03