Functional expression and characterization of five wax ester synthases in Saccharomyces cerevisiae and their utility for biodiesel production
Journal article, 2012

Background Wax ester synthases (WSs) can synthesize wax esters from alcohols and fatty acyl coenzyme A thioesters. The knowledge of the preferred substrates for each WS allows the use of yeast cells for the production of wax esters that are high-value materials and can be used in a variety of industrial applications. The products of WSs include fatty acid ethyl esters, which can be directly used as biodiesel. Results Here, heterologous WSs derived from five different organisms were successfully expressed and evaluated for their substrate preference in Saccharomyces cerevisiae. We investigated the potential of the different WSs for biodiesel (that is, fatty acid ethyl esters) production in S. cerevisiae. All investigated WSs, from Acinetobacter baylyi ADP1, Marinobacter hydrocarbonoclasticus DSM 8798, Rhodococcus opacus PD630, Mus musculus C57BL/6 and Psychrobacter arcticus 273-4, have different substrate specificities, but they can all lead to the formation of biodiesel. The best biodiesel producing strain was found to be the one expressing WS from M. hydrocarbonoclasticus DSM 8798 that resulted in a biodiesel titer of 6.3 mg/L. To further enhance biodiesel production, acetyl coenzyme A carboxylase was up-regulated, which resulted in a 30% increase in biodiesel production. Conclusions Five WSs from different species were functionally expressed and their substrate preference characterized in S. cerevisiae, thus constructing cell factories for the production of specific kinds of wax ester. WS from M. hydrocarbonoclasticus showed the highest preference for ethanol compared to the other WSs, and could permit the engineered S. cerevisiae to produce biodiesel.

Biodiesel

fatty acid ethyl esters

wax ester synthase

metabolic engineering

Saccharomyces cerevisiae

Author

Shuobo Shi

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Juan Octavio Valle Rodriguez

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Sakda Khoomrung

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Verena Siewers

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Jens B Nielsen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Biotechnology for Biofuels

1754-6834 (eISSN)

Vol. 5 Art. no. 7- 7

Driving Forces

Sustainable development

Subject Categories

Industrial Biotechnology

Areas of Advance

Energy

Life Science Engineering (2010-2018)

DOI

10.1186/1754-6834-5-7

More information

Created

10/7/2017