Enzymes Immobilized in Mesoporous Silica: a Physical-Chemical Perspective
Journal article, 2014

Mesoporous materials as support for immobilized enzymes have been explored extensively during the last two decades, primarily not only for biocatalysis applications, but also for biosensing, biofuels and enzyme-controlled drug delivery. The activity of the immobilized enzymes inside the pores is often different compared to that of the free enzymes, and an important challenge is to understand how the immobilization affects the enzymes in order to design immobilization conditions that lead to optimal enzyme activity. This review summarizes methods that can be used to understand how material properties can be linked to changes in enzyme activity. Real-time monitoring of the immobilization process and techniques that demonstrate that the enzymes are located inside the pores is discussed by contrasting them to the common practice of indirectly measuring the depletion of the protein concentration or enzyme activity in the surrounding bulk phase. We propose that pore filling (pore volume fraction occupied by proteins) is the best standard for comparing the amount of immobilized enzymes at themolecular level, and present equations to calculate pore filling from the more commonly reported immobilized mass. Methods to detect changes in enzyme structure upon immobilization and to study the microenvironment inside the pores are discussed in detail. Combining the knowledge generated from these methodologies should aid in rationally designing biocatalyst based on enzymes immobilized in mesoporous materials.

Author

Nils Carlsson

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Hanna Gustafsson

SuMo Biomaterials

Chalmers, Chemical and Biological Engineering, Applied Surface Chemistry

Christian Thörn

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Lisbeth Olsson

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Björn Åkerman

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Krister Holmberg

Chalmers, Chemical and Biological Engineering, Applied Surface Chemistry

Advances in Colloid and Interface Science

0001-8686 (ISSN)

Vol. 205 339-360

Subject Categories

Physical Chemistry

Chemical Sciences

Biocatalysis and Enzyme Technology

Areas of Advance

Materials Science

DOI

10.1016/j.cis.2013.08.010

More information

Created

10/7/2017