Enzymes Immobilized in Mesoporous Silica: a Physical-Chemical Perspective
Artikel i vetenskaplig tidskrift, 2014

Mesoporous materials as support for immobilized enzymes have been explored extensively during the last two decades, primarily not only for biocatalysis applications, but also for biosensing, biofuels and enzyme-controlled drug delivery. The activity of the immobilized enzymes inside the pores is often different compared to that of the free enzymes, and an important challenge is to understand how the immobilization affects the enzymes in order to design immobilization conditions that lead to optimal enzyme activity. This review summarizes methods that can be used to understand how material properties can be linked to changes in enzyme activity. Real-time monitoring of the immobilization process and techniques that demonstrate that the enzymes are located inside the pores is discussed by contrasting them to the common practice of indirectly measuring the depletion of the protein concentration or enzyme activity in the surrounding bulk phase. We propose that pore filling (pore volume fraction occupied by proteins) is the best standard for comparing the amount of immobilized enzymes at themolecular level, and present equations to calculate pore filling from the more commonly reported immobilized mass. Methods to detect changes in enzyme structure upon immobilization and to study the microenvironment inside the pores are discussed in detail. Combining the knowledge generated from these methodologies should aid in rationally designing biocatalyst based on enzymes immobilized in mesoporous materials.

Författare

Nils Carlsson

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Hanna Gustafsson

SuMo Biomaterials

Chalmers, Kemi- och bioteknik, Teknisk ytkemi

Christian Thörn

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

Lisbeth Olsson

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

Björn Åkerman

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Krister Holmberg

Chalmers, Kemi- och bioteknik, Teknisk ytkemi

Advances in Colloid and Interface Science

0001-8686 (ISSN)

Vol. 205 339-360

Ämneskategorier

Fysikalisk kemi

Kemi

Biokatalys och enzymteknik

Styrkeområden

Materialvetenskap

DOI

10.1016/j.cis.2013.08.010

Mer information

Skapat

2017-10-07