Björn Åkerman

Biträdande professor at Chemistry and Chemical Engineering, Chemistry and Biotechnology, Physical Chemistry

The research of the Åkerman group focuses on the behaviour of DNA and proteins in confining environments. Individual DNA molecules are subjected to mechanical and electric forces in order to understand basic principles for their biological function and analytical separation. Proteins are immobilized in mesoporous particles which are used to recycle enzymes exploited to catalyze biotechnical reactions. In both cases the spectroscopic expertise of the research group is used in close collaboration with surface chemists and molecular biologists at Chalmers.

Source: chalmers.se

Projects

2016–2019

Spectroscopic studies of an enzymatic cascade immobilized in mesoporous particles.

Björn Åkerman Physical Chemistry
Swedish Research Council (VR)

There might be more projects where Björn Åkerman participates, but you have to be logged in as a Chalmers employee to see them.

Publications

2017

Immobilization of Enzymes in Mesoporous Silica Particles: Protein Concentration and Rotational Mobility in the Pores

Pegah Sadat Nabavi Zadeh, Björn Åkerman
Journal of Physical Chemistry B. Vol. 121 (12), p. 2575-2583
Journal article
2016

Distribution of Immobilized Enzymes on the Surface and into the Mesoporous Silica Particle

Pegah Sadat Nabavi Zadeh, Björn Åkerman
Biophysical Journal. Vol. 110 (3), p. 550A-550A
Journal article
2015

A fluorescence spectroscopy assay for real-time monitoring of enzyme immobilization into mesoporous silica particles

Pegah Sadat Nabavi Zadeh, Kassam Abdel Mallak, Nils Carlsson et al
Analytical Biochemistry. Vol. 476, p. 51-58
Journal article
2014

Characterization of self-assembled DNA concatemers from synthetic oligonucleotides

Lu Sun, Björn Åkerman
Computational and Structural Biotechnology Journal. Vol. 11 (18), p. 66-72
Journal article
2014

Sensing Conformational Changes in DNA upon Ligand Binding Using QCM-D. Polyamine Condensation and Rad51 Extension of DNA Layers

Lu Sun, Karolin Frykholm, Louise Fornander et al
Journal of Physical Chemistry B. Vol. 118 (41), p. 11895-11904
Journal article
2014

Construction and Modeling of Concatemeric DNA Multilayers on a Planar Surface as Monitored by QCM-D and SPR

Lu Sun, Sofia Svedhem, Björn Åkerman
Langmuir. Vol. 30 (28), p. 8432-8441
Journal article
2014

Learning to argue as a biotechnologist: Disprivileging opposition to genetically modified food.

Anne Solli, Frank Bach, Björn Åkerman
Cultural Studies of Science Education. Vol. 9 (1), p. 1-23
Journal article
2014

Force-induced melting of DNA-evidence for peeling and internal melting from force spectra on short synthetic duplex sequences

Niklas Bosaeus, Afaf El-Sagheer, T. Brown et al
Nucleic Acids Research. Vol. 42 (12), p. 8083-8091
Journal article
2014

Enzymes Immobilized in Mesoporous Silica: a Physical-Chemical Perspective

Nils Carlsson, Hanna Gustafsson, Christian Thörn et al
Advances in Colloid and Interface Science. Vol. 205, p. 339-360
Journal article
2014

Monitoring the Kinetics of Enzyme Immobilization into Mesoporous Silica by Real Time Fluorescence

Pegah Sadat Nabavi Zadeh, Nils Carlsson, Kassam Abdel Mallak et al
Biophysical Journal. Vol. 106 (2), p. 209A-209A
Magazine article
2013

Tension Induces a Base-Paired Overstretched DNA Conformation

Niklas Bosaeus, Afaf El-Sagheer, T. Brown et al
Biophysical Journal. Vol. 104 (2), p. 165A-165A
Magazine article
2013

A method to measure pH inside mesoporous particles using protein-bound SNARF1 fluorescent probe

Christian Thörn, Nils Carlsson, Hanna Gustafsson et al
Microporous and Mesoporous Materials. Vol. 165, p. 240-246
Journal article
2013

A method to measure pH inside mesoporous particles using protein-bound SNARF1 fluorescent probe

Christian Thörn, Nils Carlsson, Hanna Gustafsson et al
Microporous and Mesoporous Materials. Vol. 165, p. 240-246
Journal article
2013

Assessing How Cationic Intercalators Affect DNA using Nanofluidic Channels

Lena Nyberg, F. Persson, Björn Åkerman et al
Biophysical Journal. Vol. 104 (2), p. 165A-
Conference poster
2013

Assessing how cationic intercalators affect DNA using nanofluidic channels

Lena Nyberg, F. Persson, Björn Åkerman et al
European Biophysics Journal. Vol. 42 (S1), p. S202-S202
Magazine article
2013

Heterogeneous staining: a tool for studies of how fluorescent dyes affect the physical properties of DNA

Lena Nyberg, Fredrik Persson, Björn Åkerman et al
Nucleic Acids Research. Vol. 41 (19), p. articlenr e184-
Journal article
2013

DNA hosted and aligned in aqueous interstitia of a lamellar liquid crystal - a membrane-biomacromolecule interaction model system

Nils Carlsson, Fabian Jonsson, Marcus Wilhelmsson et al
Soft Matter. Vol. 9 (33), p. 7951-7959
Journal article
2012

Spectroscopic characterization of Coomassie blue and its binding to amyloid fibrils

Nils Carlsson, Catherine Kitts, Björn Åkerman
Analytical Biochemistry. Vol. 420 (1), p. 33-40
Journal article
2012

Short Oligonucleotides Aligned in Stretched Humid Matrix – Secondary DNA structure in Poly(Vinyl Alcohol) Environment

Piotr Hanczyc, Björn Åkerman, Bengt Nordén
Langmuir. Vol. 28 (16), p. 6662-6669
Journal article
2012

Tension induces a base-paired overstretched DNA conformation

Niklas Bosaeus, Afaf El-Sagheer, T. Brown et al
Proceedings of the National Academy of Sciences of the United States of America. Vol. 109 (38), p. 15179-15184
Journal article
2012

Tension induces a base-paired overstretched DNA conformation

Niklas Bosaeus, Afaf El-Sagheer, T. Brown et al
Proceedings of the National Academy of Sciences of the United States of America. Vol. 109 (38), p. 15179-15184
Journal article
2012

Covalent functionalization of carbon nanotube forests grown in situ on a metal-silicon chip

Johan Johansson, Niklas Bosaeus, Nina Kann et al
Journal of Logic and Computation. Vol. 8344
Paper in proceedings
2011

Quantification of protein concentration by the Bradford method in the presence of pharmaceutical polymers

Nils Carlsson, Annika Borde, Sebastian Wölfel et al
Analytical Biochemistry. Vol. 411 (1), p. 116-121
Journal article
2011

Feruloyl esterases for biosynthesis in mesoporous silica

Christian Thörn, Nils Carlsson, Evangelos Topakas et al
Conference poster
2011

Direct probing of ion pair formation using a symmetric triangulenium dye

Fredrik Westerlund, Jonas Elm, Jacob Lykkebo et al
Photochemical and Photobiological Sciences. Vol. 10 (12), p. 1963-1973
Journal article
2011

DNA in a polyvinyl alcohol matrix and interactions with three intercalating cyanine dyes

Piotr Hanczyc, Bengt Nordén, Björn Åkerman
Journal of Physical Chemistry B. Vol. 115 (42), p. 12192-12201
Journal article
2010

Structures of self-assembled amphiphilic peptide-heterodimers: effects of concentration, pH, temperature and ionic strength

Z. L. Luo, Björn Åkerman, S. G. Zhang et al
Soft Matter. Vol. 6 (10), p. 2260-2270
Journal article
2007

Binding of Intercalating and Groove-Binding Cyanine Dyes to Bacteriophage T5

Maja Eriksson, Maria Härdelin, Anette Larsson et al
Journal of Physical Chemistry B. Vol. 111 (5), p. 1139-1148
Journal article
2007

Electrophoretic transport of latex particles in lipid nanotubes

Michal Tokarz, Bodil Hakonen, P. Dommersnes et al
Langmuir. Vol. 23 (14), p. 7652-7658
Journal article
2006

Comparison of oligonucleotide mirgation in a bicontinuous cubic phase of monoolein and water and in a fibrous agarose hydrogel

Nima Sanandaji, Nils Carlsson, Marina Voinova et al
Electrophoresis. Vol. 27 (15), p. 3007-3017
Journal article
2006

Bicontinuous cubic phase of monoolein and water as medium for electrophoresis of both membrane-bound probes and DNA

Nils Carlsson, Nima Sanandaji, Marina Voinova et al
Langmuir. Vol. 22 (9), p. 4408-4414
Journal article
2006

Single-molecule measurements of trapped and migrating circular DNA during electrophoresis in agarose gels

K. D. Cole, A. Gaigalas, Björn Åkerman
Electrophoresis. Vol. 27 (22), p. 4396-4407
Journal article
2006

Comparing mono- and divalent DNA groove binding cyanine dyes--Binding geometries, dissociation rates, and fluorescence properties

Maja Eriksson, Fredrik Westerlund, Merima Hasani et al
Biophysical Chemistry. Vol. 122 (3), p. 195-205
Journal article
2005

Electrophoretic properties of complexes between DNA and the cationic surfactant cetyltrimethylammonium bromide

R. S. Dias, R. Svingen, B. Gustavsson et al
Electrophoresis. Vol. 26 (15), p. 2908-2917
Journal article
2005

Diamond cubic phase of monoolein and water as an amphiphilic matrix for electrophoresis of oligonucleotides

Nils Carlsson, AS Winge, Sven Engström et al
JOURNAL OF PHYSICAL CHEMISTRY B. Vol. 109 (39), p. 18628-18636
Journal article
2005

Single-file electrophoretic transport and counting of individual DNA molecules in surfactant nanotubes

Michal Tokarz, Björn Åkerman, Jessica Olofsson et al
Proceedings of the National Academy of Sciences of the United States of America. Vol. 102 (26), p. 9127-9132
Journal article
2005

Time-resolved electrophoretic analysis of mobility shifts for dissociating DNA ligands

M. Eriksson, Merima Hasani, Gunnar Westman et al
Electrophoresis. Vol. 26 (3), p. 524-532
Journal article
1998

Binding Mode of Norfloxacin to Calf Thymus DNA

G.S. Son, J-A. Yeo, M-S. Kim et al
Journal of the American Chemical Society. Vol. 120 (26), p. 6451-6457
Journal article
1996

DAPI staining of DNA: Effects of charge, flexibility and contour length on orientational dynamics and mobility of DNA during agarose gel elctrophoresis

Anette Larsson, Björn Åkerman, Mats Jonsson
Journal of Chemical Physics. Vol. 100, p. 3252-
Journal article
1996

DNA Interactions with Substitution-Inert Transition Metal Ion Complexes

Bengt Nordén, Per Lincoln, Björn Åkerman et al
Probing of nucleic acids by metal ion complexes of small molecules (Metal ions in biological systems. Vol. 33), p. 177-252
Chapter in monograph, book - peer reviewed
1995

Period times and helix alignment during the cyclic migration of DNA in electrophoreses gels studied by fluorescence microscopy

Anette Larsson, Björn Åkerman
Macromolecules. Vol. 28, p. 4441-
Journal article
1991

ELECTROPHORETIC ORIENTATION OF DNA

Bengt Nordén, Christer Elvingson, Mats Jonsson et al
FIRST INTERNATIONAL CONFERENCE ON ELECTROPHORESIS, SUPERCOMPUTING AND THE HUMAN GENOME, p. 173-198
Chapter in monograph, book - peer reviewed
1991

MICROSCOPIC BEHAVIOR OF DNA DURING ELECTROPHORESIS - ELECTROPHORETIC ORIENTATION

Bengt Nordén, Christer Elvingson, Mats Jonsson et al
Quarterly Reviews of Biophysics. Vol. 24 (2), p. 103-164
Journal article
1989

ORIENTATIONAL DYNAMICS OF T2 DNA DURING AGAROSE-GEL ELECTROPHORESIS - INFLUENCE OF GEL CONCENTRATION AND ELECTRIC-FIELD STRENGTH

Björn Åkerman, Mats Jonsson, Bengt Nordén et al
Biopolymers. Vol. 28 (9), p. 1541-1571
Journal article
1989

Characterization of the Electronic-Structure of 4',6-Diamidino-2-Phenylindole

Mikael Kubista, Björn Åkerman, Bo Albinsson
Journal of the American Chemical Society. Vol. 111 (18), p. 7031-7035
Journal article
1988

New Techniques for Aligning Molecules:

Bengt Nordén, Mats Jonsson, Björn Åkerman et al
Polarized spectroscopy of ordered systems; edited by B. Samori' and E.W. Thulstrup (Proceedings of the NATO Advanced Study Institute on New Developments in Polarized Spectroscopy of Ordered Systems, Rimini, Italy, October 11-23, 1987), p. 197-209
Conference contribution
1988

Orientation of DNA during gel electrophoresis studied with linear dichroism spectroscopy

Mats Jonsson, Björn Åkerman, Bengt Nordén
Biopolymers. Vol. 27 (3), p. 381-414
Journal article
1988

Induced circular dichroism in nonintercalative DNA-drug complexes: sector rules for structural applications

Mikael Kubista, Björn Åkerman, Bengt Nordén
Journal of Physical Chemistry. Vol. 92 (8), p. 2352-2356
Journal article
1988

Characterization of the Spectroscopic Properties of Aromatic-Molecules by Polarized Spectroscopy

Mikael Kubista, Bo Albinsson, Björn Åkerman et al
Finnish Chemical Letters. Vol. 15 (3-4), p. 38-39
Journal article
1987

Characterization of interaction between DNA and 4',6-diamidino-2-phenylindole by optical spectroscopy

Mikael Kubista, Björn Åkerman, Bengt Nordén
Biochemistry. Vol. 26 (14), p. 4545-4553
Journal article
1985

Electrophoretic orientation of DNA detected by linear dichroism spectroscopy

Björn Åkerman, Mats Jonsson, Bengt Nordén
Journal of the Chemical Society - Series Chemical Communications (7), p. 422-423
Journal article