Seed culture pre-adaptation of Bacillus coagulans MA-13 improves lactic acid production in simultaneous saccharification and fermentation
Journal article, 2019

Background
Lignocellulosic biomass is an abundant and sustainable feedstock, which represents a promising raw material for the production of lactic acid via microbial fermentation. However, toxic compounds that affect microbial growth and metabolism are released from the biomass upon thermochemical pre-treatment. So far, susceptibility of bacterial strains to biomass-derived inhibitors still represents a major barrier to lactic acid production from lignocellulose. Detoxification of the pre-treated lignocellulosic material by water washing is commonly performed to alleviate growth inhibition of the production microorganism and achieve higher production rates.
Results
In this study, we assessed the feasibility of replacing the washing step with integrated cellular adaptation during pre-culture of Bacillus coagulans MA-13 prior to simultaneous saccharification and lactic acid fermentation of steam exploded wheat straw. Using a seed culture pre-exposed to 30% hydrolysate led to 50% shorter process time, 50% higher average volumetric and 115% higher average specific productivity than when using cells from a hydrolysate-free seed culture.
Conclusions
Pre-exposure of B. coagulans MA-13 to hydrolysate supports adaptation to the actual production medium. This strategy leads to lower process water requirements and combines cost-effective seed cultivation with physiological pre-adaptation of the production strain, resulting in reduced lactic acid production costs.

Hydrolysate

Simultaneous saccharification and fermentation

Bacillus coagulans

Wheat straw

Pre-adaptation

Author

Martina Aulitto

Universita degli Studi di Napoli Federico II

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Salvatore Fusco

Universita degli Studi di Napoli Federico II

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

David Benjamin Nickel

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Simonetta Bartolucci

Universita degli Studi di Napoli Federico II

Patrizia Contursi

Universita degli Studi di Napoli Federico II

Carl Johan Franzén

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Biotechnology for Biofuels

1754-6834 (eISSN)

Vol. 12 1 45

Bacillus coagulans as microbial cell factory for L-lactic acid production from agricultural and forestry waste

Stiftelsen BLANCEFLOR Boncompagni Ludovisi, född Bildt, -- .

Upgrading of renewable domestic raw materials to value-added bulk and fine chemicals for a biobased economy: technology development, systems integration and environmental impact assessment (BioBuF)

Västra Götalandsregionen, Regional development committee, 2013-11-01 -- 2018-10-31.

Formas, 2013-06-17 -- 2018-12-31.

Driving Forces

Sustainable development

Subject Categories

Biochemicals

Industrial Biotechnology

Bioprocess Technology

Areas of Advance

Energy

Life Science Engineering (2010-2018)

DOI

10.1186/s13068-019-1382-2

More information

Latest update

7/15/2019