Probing physical properties of DNA-protein complexes using nanofluidic channels
Paper in proceedings, 2014

We present the use of nanofluidic channels as a tool for determining physical properties of single DNA-protein complexes. By coating the nanochannels with a lipid bilayer we avoid sticking of proteins to the channel walls. RecA is a prokaryotic protein involved in recombination and DNA repair. We study filaments of RecA, bound to both double stranded (ds) and single stranded (ss) DNA. We determine the persistence length of RecA filaments on both dsDNA and ssDNA and obtain values in agreement with the literature. Neither the DNA nor the protein has to be attached to handles or surfaces, and the technique is directly transferable to Lab-on-a-Chip technologies for high throughput measurements in solution.

Lab-on-a-Chip

Nanofluidics

Single DNA molecules

DNA-protein interactions

Author

Karolin Frykholm

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Mohammadreza Alizadehheidari

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Louise Fornander

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Joachim Fritzsche

Chalmers, Applied Physics, Chemical Physics

Jens Wigenius

Chalmers, Chemical and Biological Engineering, Physical Chemistry

P. J. Beuning

M. Modesti

F. Persson

Fredrik Westerlund

Chalmers, Chemical and Biological Engineering, Physical Chemistry

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013

Vol. 2 1311-1313

Areas of Advance

Nanoscience and Nanotechnology (2010-2017)

Life Science Engineering (2010-2018)

Subject Categories

Physical Chemistry

Nano Technology

ISBN

978-163266624-6

More information

Created

10/8/2017