Probing physical properties of DNA-protein complexes using nanofluidic channels
Paper in proceeding, 2013

We present the use of nanofluidic channels as a tool for determining physical properties of single DNA-protein complexes. By coating the nanochannels with a lipid bilayer we avoid sticking of proteins to the channel walls. RecA is a prokaryotic protein involved in recombination and DNA repair. We study filaments of RecA, bound to both double stranded (ds) and single stranded (ss) DNA. We determine the persistence length of RecA filaments on both dsDNA and ssDNA and obtain values in agreement with the literature. Neither the DNA nor the protein has to be attached to handles or surfaces, and the technique is directly transferable to Lab-on-a-Chip technologies for high throughput measurements in solution.

DNA-protein interactions

Single DNA molecules




Karolin Frykholm

Chemical Biology

Mohammadreza Alizadehheidari

Chemical Biology

Louise Fornander

Physical Chemistry

Joachim Fritzsche

Chalmers, Applied Physics, Chemical Physics

Jens Wigenius

Physical Chemistry

P. J. Beuning

Northeastern University

M. Modesti

Aix Marseille University

F. Persson

Uppsala University

Fredrik Westerlund

Physical Chemistry

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013

Vol. 2 1311-1313
978-163266624-6 (ISBN)

Areas of Advance

Nanoscience and Nanotechnology

Life Science Engineering (2010-2018)

Subject Categories

Physical Chemistry

Nano Technology

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