Probing physical properties of DNA-protein complexes using nanofluidic channels
Paper i proceeding, 2014

We present the use of nanofluidic channels as a tool for determining physical properties of single DNA-protein complexes. By coating the nanochannels with a lipid bilayer we avoid sticking of proteins to the channel walls. RecA is a prokaryotic protein involved in recombination and DNA repair. We study filaments of RecA, bound to both double stranded (ds) and single stranded (ss) DNA. We determine the persistence length of RecA filaments on both dsDNA and ssDNA and obtain values in agreement with the literature. Neither the DNA nor the protein has to be attached to handles or surfaces, and the technique is directly transferable to Lab-on-a-Chip technologies for high throughput measurements in solution.

Lab-on-a-Chip

Nanofluidics

Single DNA molecules

DNA-protein interactions

Författare

Karolin Frykholm

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Mohammadreza Alizadehheidari

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Louise Fornander

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Joachim Fritzsche

Chalmers, Teknisk fysik, Kemisk fysik

Jens Wigenius

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

P. J. Beuning

M. Modesti

F. Persson

Fredrik Westerlund

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013

Vol. 2 1311-1313

Styrkeområden

Nanovetenskap och nanoteknik

Livsvetenskaper och teknik

Ämneskategorier

Fysikalisk kemi

Nanoteknik

ISBN

978-163266624-6