Optical mapping of single DNA molecules in nanochannels: A novel method for identification and characterization of antibiotic resistance
Paper in proceeding, 2015

The use, and overuse, of antibiotics has during the last decade led to a dramatic increase in antibiotic resistance and there is a crying need for novel methods for fast identification of antibiotic resistance genes. We here demonstrate how our previously developed assay for optical mapping of DNA in nanochannels can be used for characterization of resistance genes located on plasmid DNA from bacteria. The assay requires extremely small sample volumes and does neither rely on PCR, nor culturing of bacteria, which greatly reduces the time for analysis.

Plasmid DNA


Antibiotic resistance

Optical mapping


Lena Nyberg

Chalmers, Biology and Biological Engineering, Chemical Biology

Gustav Emilsson

Chalmers, Applied Physics, Bionanophotonics

A. Nilsson

Lund University

E. Lagerstedt

Lund University

C. Noble

Lund University

Liselott Svensson-Stadler

University of Gothenburg

N. Karami

University of Gothenburg

Fei Sjöberg

University of Gothenburg

Edward R.B. Moore

University of Gothenburg

Joachim Fritzsche

Chalmers, Applied Physics, Chemical Physics

Erik Kristiansson

University of Gothenburg

T. Ambjornsson

Lund University

Fredrik Westerlund

Chalmers, Biology and Biological Engineering, Chemical Biology

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

978-09-79-80647-6 (ISBN)

Areas of Advance

Nanoscience and Nanotechnology

Life Science Engineering (2010-2018)

Subject Categories

Biochemistry and Molecular Biology



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