Optical mapping of single DNA molecules in nanochannels: A novel method for identification and characterization of antibiotic resistance
Paper in proceedings, 2015

The use, and overuse, of antibiotics has during the last decade led to a dramatic increase in antibiotic resistance and there is a crying need for novel methods for fast identification of antibiotic resistance genes. We here demonstrate how our previously developed assay for optical mapping of DNA in nanochannels can be used for characterization of resistance genes located on plasmid DNA from bacteria. The assay requires extremely small sample volumes and does neither rely on PCR, nor culturing of bacteria, which greatly reduces the time for analysis.

Plasmid DNA

Nanofluidics

Antibiotic resistance

Optical mapping

Author

Lena Nyberg

Chalmers, Biology and Biological Engineering, Chemical Biology

Gustav Emilsson

Chalmers, Applied Physics, Bionanophotonics

A. Nilsson

Lund University

E. Lagerstedt

Lund University

C. Noble

Lund University

Liselott Svensson-Stadler

University of Gothenburg

N. Karami

University of Gothenburg

Fei Sjöberg

University of Gothenburg

Edward R.B. Moore

University of Gothenburg

Joachim Fritzsche

Chalmers, Applied Physics, Chemical Physics

Erik Kristiansson

University of Gothenburg

T. Ambjornsson

Lund University

Fredrik Westerlund

Chalmers, Biology and Biological Engineering, Chemical Biology

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

1045-1047

Areas of Advance

Nanoscience and Nanotechnology (2010-2017)

Life Science Engineering (2010-2018)

Subject Categories

Biochemistry and Molecular Biology

ISBN

978-09-79-80647-6

More information

Latest update

3/2/2018 9