Optical mapping of single DNA molecules in nanochannels: A novel method for identification and characterization of antibiotic resistance

Lena Nyberg; Gustav Emilsson; A. Nilsson; E. Lagerstedt; C. Noble; Liselott Svensson-Stadler; N. Karami; Fei Sjöberg; Edward R.B. Moore; Joachim Fritzsche; Erik Kristiansson; T. Ambjornsson; Fredrik Westerlund

Optical mapping of single DNA molecules in nanochannels: A novel method for identification and characterization of antibiotic resistance
Paper i proceeding, 2015

The use, and overuse, of antibiotics has during the last decade led to a dramatic increase in antibiotic resistance and there is a crying need for novel methods for fast identification of antibiotic resistance genes. We here demonstrate how our previously developed assay for optical mapping of DNA in nanochannels can be used for characterization of resistance genes located on plasmid DNA from bacteria. The assay requires extremely small sample volumes and does neither rely on PCR, nor culturing of bacteria, which greatly reduces the time for analysis.

Plasmid DNA

Nanofluidics

Antibiotic resistance

Optical mapping

Författare

Lena Nyberg

Chalmers, Biologi och bioteknik, Kemisk biologi

Forskning Andra publikationer

Gustav Emilsson

Chalmers, Teknisk fysik, Bionanofotonik

Forskning Andra publikationer

A. Nilsson

Lunds universitet

E. Lagerstedt

Lunds universitet

C. Noble

Lunds universitet

Liselott Svensson-Stadler

Göteborgs universitet

N. Karami

Göteborgs universitet

Fei Sjöberg

Göteborgs universitet

Edward R.B. Moore

Göteborgs universitet

Joachim Fritzsche

Chalmers, Teknisk fysik, Kemisk fysik

Forskning Andra publikationer

Erik Kristiansson

Göteborgs universitet

Forskning Andra publikationer

T. Ambjornsson

Lunds universitet

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi

Forskning Andra publikationer

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

1045-1047
978-09-79-80647-6 (ISBN)

Styrkeområden

Nanovetenskap och nanoteknik

Livsvetenskaper och teknik (2010-2018)

Ämneskategorier

Biokemi och molekylärbiologi

ISBN

978-09-79-80647-6

Mer information

Senast uppdaterat

2018-03-02

Optical mapping of single DNA molecules in nanochannels: A novel method for identification and characterization of antibiotic resistance Paper i proceeding, 2015

Författare

Lena Nyberg

Gustav Emilsson

A. Nilsson

E. Lagerstedt

C. Noble

Liselott Svensson-Stadler

N. Karami

Fei Sjöberg

Edward R.B. Moore

Joachim Fritzsche

Erik Kristiansson

T. Ambjornsson

Fredrik Westerlund

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Styrkeområden

Ämneskategorier

ISBN

Mer information

Senast uppdaterat

Optical mapping of single DNA molecules in nanochannels: A novel method for identification and characterization of antibiotic resistance
Paper i proceeding, 2015