Light-sensing via hydrogen peroxide and a peroxiredoxin
Journal article, 2017
Yeast lacks dedicated photoreceptors; however, blue light still causes pronounced oscillations of the transcription factor Msn2 into and out of the nucleus. Here we show that this poorly understood phenomenon is initiated by a peroxisomal oxidase, which converts light into a hydrogen peroxide (H2O2) signal that is sensed by the peroxiredoxin Tsa1 and transduced to thioredoxin, to counteract PKA-dependent Msn2 phosphorylation. Upon H2O2, the nuclear retention of PKA catalytic subunits, which contributes to delayed Msn2 nuclear concentration, is antagonized in a Tsa1-dependent manner. Conversely, peroxiredoxin hyperoxidation interrupts the H2O2 signal and drives Msn2 oscillations by superimposing on PKA feedback regulation. Our data identify a mechanism by which light could be sensed in all cells lacking dedicated photoreceptors. In particular, the use of H2O2 as a second messenger in signalling is common to Msn2 oscillations and to light-induced entrainment of circadian rhythms and suggests conserved roles for peroxiredoxins in endogenous rhythms.
nuclear-localization
yeast transcription factor
saccharomyces-cerevisiae
factor msn2p
gene disruption
protein-kinase
life-span
circadian-rhythms
core component
glucose starvation
Author
Kristofer Bodvard
Chalmers, Physics, Bionanophotonics
K. Peeters
University of Gothenburg
F. Roger
University of Gothenburg
N. Romanov
European Molecular Biology Laboratory
University of Vienna
A. Igbaria
University of Paris-Sud
Niek Welkenhuysen
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
G. Palais
University of Paris-Sud
W. Reiter
University of Vienna
M. B. Toledano
University of Paris-Sud
Mikael Käll
Chalmers, Physics, Bionanophotonics
M. Molin
University of Gothenburg
Nature Communications
2041-1723 (ISSN) 20411723 (eISSN)
Vol. 8 14791Subject Categories
Biochemistry and Molecular Biology
Cell and Molecular Biology
Other Physics Topics
DOI
10.1038/ncomms14791
PubMed
28337980