Light-sensing via hydrogen peroxide and a peroxiredoxin
Artikel i vetenskaplig tidskrift, 2017

Yeast lacks dedicated photoreceptors; however, blue light still causes pronounced oscillations of the transcription factor Msn2 into and out of the nucleus. Here we show that this poorly understood phenomenon is initiated by a peroxisomal oxidase, which converts light into a hydrogen peroxide (H2O2) signal that is sensed by the peroxiredoxin Tsa1 and transduced to thioredoxin, to counteract PKA-dependent Msn2 phosphorylation. Upon H2O2, the nuclear retention of PKA catalytic subunits, which contributes to delayed Msn2 nuclear concentration, is antagonized in a Tsa1-dependent manner. Conversely, peroxiredoxin hyperoxidation interrupts the H2O2 signal and drives Msn2 oscillations by superimposing on PKA feedback regulation. Our data identify a mechanism by which light could be sensed in all cells lacking dedicated photoreceptors. In particular, the use of H2O2 as a second messenger in signalling is common to Msn2 oscillations and to light-induced entrainment of circadian rhythms and suggests conserved roles for peroxiredoxins in endogenous rhythms.

yeast transcription factor

protein-kinase

core component

nuclear-localization

factor msn2p

glucose starvation

circadian-rhythms

saccharomyces-cerevisiae

life-span

gene disruption

Författare

Kristofer Bodvard

Chalmers, Fysik, Bionanofotonik

K. Peeters

Göteborgs universitet

F. Roger

Göteborgs universitet

N. Romanov

European Molecular Biology Laboratory

Universität Wien

A. Igbaria

Université Paris-Sud

Niek Welkenhuysen

Chalmers, Biologi och bioteknik, Systembiologi

G. Palais

Université Paris-Sud

W. Reiter

Universität Wien

M. B. Toledano

Université Paris-Sud

Mikael Käll

Chalmers, Fysik, Bionanofotonik

M. Molin

Göteborgs universitet

Nature Communications

2041-1723 (ISSN)

Vol. 8 14791

Ämneskategorier

Cellbiologi

Biokemi och molekylärbiologi

Cell- och molekylärbiologi

DOI

10.1038/ncomms14791

PubMed

28337980

Mer information

Senast uppdaterat

2018-08-01