Interlaboratory coverage test on plant food bioactive compounds and their metabolites by mass spectrometry-based untargeted metabolomics
Journal article, 2018
Bioactive compounds present in plant-based foods, and their metabolites derived from gut microbiota and endogenous metabolism, represent thousands of chemical structures of potential interest for human nutrition and health. State-of-the-art analytical methodologies, including untargeted metabolomics based on high-resolution mass spectrometry, are required for the profiling of these compounds in complex matrices, including plant food materials and biofluids. The aim of this project was to compare the analytical coverage of untargeted metabolomics methods independently developed and employed in various European platforms. In total, 56 chemical standards representing the most common classes of bioactive compounds spread over a wide chemical space were selected and analyzed by the participating platforms (n = 13) using their preferred untargeted method. The results were used to define analytical criteria for a successful analysis of plant food bioactives. Furthermore, they will serve as a basis for an optimized consensus method.
Method development
Mass spectrometry
Phytochemicals
Author
Ville Mikael Koistinen
University of Eastern Finland
Andreia Bento da Silva
Nova University of Lisbon
László Abrankó
Szent István University
Dorrain Low
Clermont Auvergne University
Rocio Garcia Villalba
Spanish National Research Council (CSIC)
Francisco Tomás Barberán
Spanish National Research Council (CSIC)
Rikard Landberg
Chalmers, Biology and Biological Engineering, Food and Nutrition Science
Otto Savolainen
Chalmers, Biology and Biological Engineering, Food and Nutrition Science
Inmaculada Alvarez-Acero
Spanish National Research Council (CSIC)
Sonia de Pascual-Teresa
Spanish National Research Council (CSIC)
Christof Van Poucke
Flanders Research Institute for Agriculture Fisheries and Food (ILVO),
Conceição Almeida
Nova University of Lisbon
Lucie Petrásková
Czech Academy of Sciences
Kateřina Valentová
Czech Academy of Sciences
Stephanie Durand
Clermont Auvergne University
Wiesław Wiczkowski
Polish Academy of Sciences
Dorota Szawara-Nowak
Polish Academy of Sciences
Raúl González-Domínguez
University of Barcelona
Institute of Health Carlos III
Technical Research Centre of Finland (VTT)
Rafael Llorach
Technical Research Centre of Finland (VTT)
Institute of Health Carlos III
University of Barcelona
Cristina Andres-Lacueva
Institute of Health Carlos III
University of Barcelona
Technical Research Centre of Finland (VTT)
Anna-Marja Aura
Instituto de Biologia Experimental e Tecnologica
Tuulikki Seppänen-Laakso
Instituto de Biologia Experimental e Tecnologica
K. Hanhineva
University of Eastern Finland
Claudine Manach
Clermont Auvergne University
Maria Rosário Bronze
Nova University of Lisbon
University of Lisbon
Metabolites
2218-1989 (ISSN) 22181989 (eISSN)
Vol. 8 3 46Subject Categories (SSIF 2011)
Analytical Chemistry
Food Science
Environmental Sciences
DOI
10.3390/metabo8030046