Simplified Intestinal Microbiota to Study Microbe-Diet-Host Interactions in a Mouse Model
Journal article, 2019
The gut microbiota can modulate human metabolism through interactions with macronutrients. However, microbiota-diet-host interactions are difficult to study because bacteria interact in complex food webs in concert with the host, and many of the bacteria are not yet characterized. To reduce the complexity, we colonize mice with a simplified intestinal microbiota (SIM) composed of ten sequenced strains isolated from the human gut with complementing pathways to metabolize dietary fibers. We feed the SIM mice one of three diets (chow [fiber rich], high-fat/high-sucrose, or zero-fat/high-sucrose diets [both low in fiber]) and investigate (1) how dietary fiber, saturated fat, and sucrose affect the abundance and transcriptome of the SIM community, (2) the effect of microbe-diet interactions on circulating metabolites, and (3) how microbiota-diet interactions affect host metabolism. Our SIM model can be used in future studies to help clarify how microbiota-diet interactions contribute to metabolic diseases.
metabolome
microbiota
diet
transcriptome
Author
P. Kovatcheva-Datchary
Wallenberg Lab.
Saeed Shoaie
King's College London
Sunjae Lee
King's College London
Annika Wahlström
Wallenberg Lab.
Intawat Nookaew
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
Anna Hallen
Wallenberg Lab.
Rosie Perkins
Wallenberg Lab.
Jens Christian Froslev Nielsen
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
Technical University of Denmark (DTU)
Fredrik Bäckhed
University of Copenhagen
Wallenberg Lab.
Cell Reports
22111247 (eISSN)
Vol. 26 13 3772-3783.e6Subject Categories
Microbiology
Microbiology in the medical area
Nutrition and Dietetics
DOI
10.1016/j.celrep.2019.02.090