Simplified Intestinal Microbiota to Study Microbe-Diet-Host Interactions in a Mouse Model
Artikel i vetenskaplig tidskrift, 2019

The gut microbiota can modulate human metabolism through interactions with macronutrients. However, microbiota-diet-host interactions are difficult to study because bacteria interact in complex food webs in concert with the host, and many of the bacteria are not yet characterized. To reduce the complexity, we colonize mice with a simplified intestinal microbiota (SIM) composed of ten sequenced strains isolated from the human gut with complementing pathways to metabolize dietary fibers. We feed the SIM mice one of three diets (chow [fiber rich], high-fat/high-sucrose, or zero-fat/high-sucrose diets [both low in fiber]) and investigate (1) how dietary fiber, saturated fat, and sucrose affect the abundance and transcriptome of the SIM community, (2) the effect of microbe-diet interactions on circulating metabolites, and (3) how microbiota-diet interactions affect host metabolism. Our SIM model can be used in future studies to help clarify how microbiota-diet interactions contribute to metabolic diseases.






P. Kovatcheva-Datchary

Wallenberg Lab.

Saeed Shoaie

King's College London

Sunjae Lee

King's College London

Annika Wahlström

Wallenberg Lab.

Intawat Nookaew

Chalmers, Biologi och bioteknik, Systembiologi

Anna Hallen

Wallenberg Lab.

Rosie Perkins

Wallenberg Lab.

Jens Christian Froslev Nielsen

Chalmers, Biologi och bioteknik, Systembiologi

Danmarks Tekniske Universitet (DTU)

Fredrik Bäckhed

Köpenhamns universitet

Wallenberg Lab.

Cell Reports

22111247 (eISSN)

Vol. 26 13 3772-3783.e6



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