Strategies and challenges for metabolic rewiring
Review article, 2019
Metabolic engineering is often centred on rewiring cellular metabolism to improve the production of chemicals. Turning cells into efficient factories is challenging because of ubiquitous and tightly regulated metabolic interactions. Tools and strategies from different disciplines, including systems biology, synthetic biology and evolutionary engineering, have been integrated into metabolic engineering to overcome challenges with rewiring metabolism for cell factory development. In this review, we summarise the recent development of tools and strategies for dynamic pathway regulation, compartmentalisation, modular and evolutionary engineering. In addition, we describe how systems biology tools benefit metabolic rewiring for advancing the development of cell factories.
Metabolic rewiring
Metabolism
Evolutionary engineering
Systems biology
Metabolic engineering
Rational engineering
Author
Tao Yu
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
Yasaman Dabirian
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
Quanli Liu
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
Verena Siewers
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
Jens Christian Froslev Nielsen
Technical University of Denmark (DTU)
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
Current Opinion in Systems Biology
24523100 (eISSN)
Vol. 15 30-38Subject Categories
Other Mechanical Engineering
Software Engineering
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
DOI
10.1016/j.coisb.2019.03.004