Interspecies plasmid transfer appears rare in sequential infections with extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae
Journal article, 2019

From a cohort of 1836 Swedish patients infected with ESBL-producing Enterobacteriaceae (EPE) during 2004–2014, 513 patients with recurrent EPE infection were identified. Only in 14 of the 513 patients was a change of species (ESBL-E. coli to ESBL-K. pneumoniae or vice versa) found between the index and subsequent infection. Eleven sequential urine isolates from 5 of the 14 patients were available for further analysis of possible transfer of ESBL-carrying plasmids. The plasmid content was studied using optical DNA mapping (ODM), PCR-based replicon typing, and ESBL gene sequencing. ODM allowed us to directly compare whole plasmids between isolates and found similar ESBL-carrying plasmids in 3 out of the 5 patients. The ODM results and the rarity in shift of species between ESBL-E. coli and ESBL-K. pneumoniae imply that in recurrent EPE infections interspecies plasmid transfer is uncommon.

Author

Anna Lindblom

University of Gothenburg

Sriram Kesarimangalam

Chalmers, Biology and Biological Engineering, Chemical Biology

Vilhelm Müller

Chalmers, Biology and Biological Engineering, Chemical Biology

Robin Öz

Chalmers, Biology and Biological Engineering, Chemical Biology

Hilda Sandström

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Physical Chemistry

Christina M. Åhrén

University of Gothenburg

Fredrik Westerlund

Chalmers, Biology and Biological Engineering, Chemical Biology

Nahid Karami

University of Gothenburg

Diagnostic Microbiology and Infectious Disease

0732-8893 (ISSN)

Vol. 93 4 380-385

Subject Categories

Infectious Medicine

Microbiology

Microbiology in the medical area

DOI

10.1016/j.diagmicrobio.2018.10.014

PubMed

30527621

More information

Latest update

5/27/2019