Interspecies plasmid transfer appears rare in sequential infections with extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae
Artikel i vetenskaplig tidskrift, 2019

From a cohort of 1836 Swedish patients infected with ESBL-producing Enterobacteriaceae (EPE) during 2004–2014, 513 patients with recurrent EPE infection were identified. Only in 14 of the 513 patients was a change of species (ESBL-E. coli to ESBL-K. pneumoniae or vice versa) found between the index and subsequent infection. Eleven sequential urine isolates from 5 of the 14 patients were available for further analysis of possible transfer of ESBL-carrying plasmids. The plasmid content was studied using optical DNA mapping (ODM), PCR-based replicon typing, and ESBL gene sequencing. ODM allowed us to directly compare whole plasmids between isolates and found similar ESBL-carrying plasmids in 3 out of the 5 patients. The ODM results and the rarity in shift of species between ESBL-E. coli and ESBL-K. pneumoniae imply that in recurrent EPE infections interspecies plasmid transfer is uncommon.


Anna Lindblom

Göteborgs universitet

Sriram Kesarimangalam

Chalmers, Biologi och bioteknik, Kemisk biologi

Vilhelm Müller

Chalmers, Biologi och bioteknik, Kemisk biologi

Robin Öz

Chalmers, Biologi och bioteknik, Kemisk biologi

Hilda Sandström

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

Christina M. Åhrén

Göteborgs universitet

Fredrik Westerlund

Chalmers, Biologi och bioteknik, Kemisk biologi

Nahid Karami

Göteborgs universitet

Diagnostic Microbiology and Infectious Disease

0732-8893 (ISSN)

Vol. 93 4 380-385




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