Nanomedical Relevance of the Intermolecular Interaction Dynamics-Examples from Lysozymes and Insulins
Journal article, 2019

Insulin and lysozyme share the common features of being prone to aggregate and having biomedical importance. Encapsulating lysozyme and insulin in micellar nanoparticles probably would prevent aggregation and facilitate oral drug delivery. Despite the vivid structural knowledge of lysozyme and insulin, the environment-dependent oligomerization (dimer, trimer, and multimer) and associated structural dynamics remain elusive. The knowledge of the intra- and intermolecular interaction profiles has cardinal importance for the design of encapsulation protocols. We have employed various biophysical methods such as NMR spectroscopy, X-ray crystallography, Thioflavin T fluorescence, and atomic force microscopy in conjugation with molecular modeling to improve the understanding of interaction dynamics during homo-oligomerization of lysozyme (human and hen egg) and insulin (porcine, human, and glargine). The results obtained depict the atomistic intra- and intermolecular interaction details of the homo-oligomerization and confirm the propensity to form fibrils. Taken together, the data accumulated and knowledge gained will further facilitate nanoparticle design and production with insulin or lysozyme-related protein encapsulation.

Author

Ruiyan Zhang

Liaocheng University

University of Kiel

RI-B-NT Research Institute of Bioinformatics and Nanotechnology

Ning Zhang

Liaocheng University

Marzieh Mohri

RI-B-NT Research Institute of Bioinformatics and Nanotechnology

Lisha Wu

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Physical Chemistry

Thomas Eckert

Justus Liebig University Giessen

University of Applied Sciences Fresenius

Vadim B. Krylov

Russian Academy of Sciences

Andrea Antosova

Slovak Academy of Sciences

Slavomira Ponikova

Slovak Academy of Sciences

Zuzana Bednarikova

Slovak Academy of Sciences

Philipp Markart

Klinikum Fulda

Justus Liebig University Giessen

Andreas Guenther

Russian Academy of Sciences

Justus Liebig University Giessen

Bengt Nordén

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry

Martin Billeter

University of Gothenburg

Roland Schauer

University of Kiel

Axel J. Scheidig

University of Kiel

Bhisma N. Ratha

Bose Institute

Anirban Bhunia

Bose Institute

Karsten Hesse

Tierarztpraxis Dr. Karsten Hesse

Mushira Abdelaziz Enani

King Fahad Medical City

Juergen Steinmeyer

Justus Liebig University Giessen

Athanasios K. Petridis

Heinrich Heine University Düsseldorf

Tibor Kozar

Pavol Jozef Safarik University

Zuzana Gazova

Slovak Academy of Sciences

Hans-Christian Siebert

RI-B-NT Research Institute of Bioinformatics and Nanotechnology

ACS OMEGA

2470-1343 (ISSN)

Vol. 4 2 4206-4220

Subject Categories

Physical Chemistry

Other Chemistry Topics

Biophysics

DOI

10.1021/acsomega.8b02471

PubMed

30847433

More information

Latest update

6/10/2019