Nanomedical Relevance of the Intermolecular Interaction Dynamics-Examples from Lysozymes and Insulins
Journal article, 2019
Insulin and lysozyme share the common features of being prone to aggregate and having biomedical importance. Encapsulating lysozyme and insulin in micellar nanoparticles probably would prevent aggregation and facilitate oral drug delivery. Despite the vivid structural knowledge of lysozyme and insulin, the environment-dependent oligomerization (dimer, trimer, and multimer) and associated structural dynamics remain elusive. The knowledge of the intra- and intermolecular interaction profiles has cardinal importance for the design of encapsulation protocols. We have employed various biophysical methods such as NMR spectroscopy, X-ray crystallography, Thioflavin T fluorescence, and atomic force microscopy in conjugation with molecular modeling to improve the understanding of interaction dynamics during homo-oligomerization of lysozyme (human and hen egg) and insulin (porcine, human, and glargine). The results obtained depict the atomistic intra- and intermolecular interaction details of the homo-oligomerization and confirm the propensity to form fibrils. Taken together, the data accumulated and knowledge gained will further facilitate nanoparticle design and production with insulin or lysozyme-related protein encapsulation.
Author
Ruiyan Zhang
University of Kiel
RI-B-NT Research Institute of Bioinformatics and Nanotechnology
Liaocheng University
Ning Zhang
Liaocheng University
Marzieh Mohri
RI-B-NT Research Institute of Bioinformatics and Nanotechnology
Lisha Wu
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
Thomas Eckert
University of Applied Sciences Fresenius
Justus Liebig University Giessen
Vadim B. Krylov
Russian Academy of Sciences
Andrea Antosova
Slovak Academy of Sciences
Slavomira Ponikova
Slovak Academy of Sciences
Zuzana Bednarikova
Slovak Academy of Sciences
Philipp Markart
Klinikum Fulda
Justus Liebig University Giessen
Andreas Guenther
Russian Academy of Sciences
Justus Liebig University Giessen
Bengt Nordén
Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry
Martin Billeter
University of Gothenburg
Roland Schauer
University of Kiel
Axel J. Scheidig
University of Kiel
Bhisma N. Ratha
Bose Institute
Anirban Bhunia
Bose Institute
Karsten Hesse
Tierarztpraxis Dr. Karsten Hesse
Mushira Abdelaziz Enani
King Fahad Medical City
Juergen Steinmeyer
Justus Liebig University Giessen
Athanasios K. Petridis
Heinrich Heine University Düsseldorf
Tibor Kozar
Pavol Jozef Safarik University
Zuzana Gazova
Slovak Academy of Sciences
Hans-Christian Siebert
RI-B-NT Research Institute of Bioinformatics and Nanotechnology
ACS Omega
24701343 (eISSN)
Vol. 4 2 4206-4220Subject Categories
Physical Chemistry
Other Chemistry Topics
Biophysics
DOI
10.1021/acsomega.8b02471
PubMed
30847433