Nanomedical Relevance of the Intermolecular Interaction Dynamics-Examples from Lysozymes and Insulins
Artikel i vetenskaplig tidskrift, 2019

Insulin and lysozyme share the common features of being prone to aggregate and having biomedical importance. Encapsulating lysozyme and insulin in micellar nanoparticles probably would prevent aggregation and facilitate oral drug delivery. Despite the vivid structural knowledge of lysozyme and insulin, the environment-dependent oligomerization (dimer, trimer, and multimer) and associated structural dynamics remain elusive. The knowledge of the intra- and intermolecular interaction profiles has cardinal importance for the design of encapsulation protocols. We have employed various biophysical methods such as NMR spectroscopy, X-ray crystallography, Thioflavin T fluorescence, and atomic force microscopy in conjugation with molecular modeling to improve the understanding of interaction dynamics during homo-oligomerization of lysozyme (human and hen egg) and insulin (porcine, human, and glargine). The results obtained depict the atomistic intra- and intermolecular interaction details of the homo-oligomerization and confirm the propensity to form fibrils. Taken together, the data accumulated and knowledge gained will further facilitate nanoparticle design and production with insulin or lysozyme-related protein encapsulation.

Författare

Ruiyan Zhang

Liaocheng University

Christian-Albrechts-Universität zu Kiel

RI-B-NT Research Institute of Bioinformatics and Nanotechnology

Ning Zhang

Liaocheng University

Marzieh Mohri

RI-B-NT Research Institute of Bioinformatics and Nanotechnology

Lisha Wu

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

Thomas Eckert

Justus Liebig University Giessen

Hochschule Fresenius

Vadim B. Krylov

Russian Academy of Sciences

Andrea Antosova

Slovenská akadémia Vied

Slavomira Ponikova

Slovenská akadémia Vied

Zuzana Bednarikova

Slovenská akadémia Vied

Philipp Markart

Klinikum Fulda

Justus Liebig University Giessen

Andreas Guenther

Russian Academy of Sciences

Justus Liebig University Giessen

Bengt Nordén

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Martin Billeter

Göteborgs universitet

Roland Schauer

Christian-Albrechts-Universität zu Kiel

Axel J. Scheidig

Christian-Albrechts-Universität zu Kiel

Bhisma N. Ratha

Bose Institute

Anirban Bhunia

Bose Institute

Karsten Hesse

Tierarztpraxis Dr. Karsten Hesse

Mushira Abdelaziz Enani

King Fahad Medical City

Juergen Steinmeyer

Justus Liebig University Giessen

Athanasios K. Petridis

Heinrich Heine Universität Düsseldorf

Tibor Kozar

Univerzita Pavla Jozefa Šafárika

Zuzana Gazova

Slovenská akadémia Vied

Hans-Christian Siebert

RI-B-NT Research Institute of Bioinformatics and Nanotechnology

ACS OMEGA

2470-1343 (ISSN)

Vol. 4 2 4206-4220

Ämneskategorier

Fysikalisk kemi

Annan kemi

Biofysik

DOI

10.1021/acsomega.8b02471

PubMed

30847433

Mer information

Senast uppdaterat

2019-06-10