Isolation, identification, and selection of strains as candidate probiotics and starters for fermentation of Swedish legumes
Journal article, 2020
cultures to substituting raw materials needs to be developed.
Objective: Aims of this study were to isolate, identify, and phenotypically characterize lactic acid bacteria (LAB) that inhabit Swedish legumes, and assess properties necessary for selecting strains with the ability to ferment a bean beverage and with potential health beneficial properties.
Design: Isolates of presumed LAB were obtained from legumes collected at Öland, Sweden. Strain diversity was assessed by repetitive polymerase chain reaction (rep-PCR). The strains were identified using matrix- assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF MS). Species belonging to Enterococcus were predominant along with Pediococcus and closely related Bacillus. Strains were tested for
tolerance to low pH, phenol, and bile as well as their bile salt hydrolase (BSH) activity. In addition, Enterococcus strains were tested for antibiotic resistance, and Pediococcus strains for their ability to ferment a bean beverage.
Results: From the 25 strains char acterized, five were found resistant to low pH, bile, and phenol, suggesting that they can survive a passage through the gastrointestinal tract (GIT) and hence potentially exert beneficial effects in the host. These are suggested for further investigation on specific host-beneficial properties. Two of these, belonging to Pediococ cus pentosaceus, were able to ferment a bean beverage without any added nutrients, indicating that the Pediococcus strains are well adapted to the bean substrate. One of the P. pentosaceus strains were also able to markedly improve the reduction of phytate by the phytase-producing yeast strain Pichia kudriavzevii TY1322 during co-fermentation as well as increase the final cell count of the yeast strain.
Conclusion: Strain isolation and characterization performed in this study aids in selecting starter cultures for legume fermentation. Nutritional properties can be improved by co-fermentation with yeast indicating that novel nutritious fermented non-dairy products could be developed.
fava bean
isolation
fermentation
pediococcus pentosaceus
phytate
Author
Cecilia Mayer Labba
Chalmers, Biology and Biological Engineering, Food and Nutrition Science
Thomas Andlid
Chalmers, Biology and Biological Engineering, Food and Nutrition Science
Åsa Lindgren
University of Gothenburg
Ann-Sofie Sandberg
Chalmers, Biology and Biological Engineering, Food and Nutrition Science
Fei Sjöberg
University of Gothenburg
Food and Nutrition Research
1654-6628 (ISSN) 1654-661X (eISSN)
Vol. 64 4410-13 4410Driving Forces
Sustainable development
Areas of Advance
Health Engineering
Life Science Engineering (2010-2018)
Subject Categories
Agricultural Biotechnology
Nutrition and Dietetics
DOI
10.29219/fnr.v64.4410