Proteome reallocation from amino acid biosynthesis to ribosomes enables yeast to grow faster in rich media
Journal article, 2020
Several recent studies have shown that the concept of proteome constraint, i.e., the need for the cell to balance allocation of its proteome between different cellular processes, is essential for ensuring proper cell function. However, there have been no attempts to elucidate how cells' maximum capacity to grow depends on protein availability for different cellular processes. To experimentally address this, we cultivated Saccharomyces cerevisiae in bioreactors with or without amino acid supplementation and performed quantitative proteomics to analyze global changes in proteome allocation, during both anaerobic and aerobic growth on glucose. Analysis of the proteomic data implies that proteome mass is mainly reallocated from amino acid biosynthetic processes into translation, which enables an increased growth rate during supplementation. Similar findings were obtained from both aerobic and anaerobic cultivations. Our findings show that cells can increase their growth rate through increasing its proteome allocation toward the protein translational machinery.
amino acid metabolism
metabolic engineering
protein translation
systems biology
Author
Johan Björkeroth
Novo Nordisk Foundation
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
Kate Campbell
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
Novo Nordisk Foundation
Carl Malina
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
Rosemary Yu
Novo Nordisk Foundation
Francesca Di Bartolomeo
SINTEF Industry
Novo Nordisk Foundation
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
Jens B Nielsen
Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology
BioInnovation Institute
Novo Nordisk Foundation
Proceedings of the National Academy of Sciences of the United States of America
0027-8424 (ISSN) 1091-6490 (eISSN)
Vol. 117 35 21804-21812Subject Categories
Cell Biology
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Other Industrial Biotechnology
DOI
10.1073/pnas.1921890117
PubMed
32817546